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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 925 results
1

TSKgel™ SP-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

For the separation and analysis of proteins, peptides, and other biologically active molecules.

Solvents ≤ 20% Organic Solvent
Stationary Phase Sulfonic Acid
Temperature 10°C to 45°C
Matrix Sulfonic Acid Phase/Hydroxylated Methacrylic Polymer
Ion Exchange Group Na+
pH 2 to 12
Technique Cation Exchange
Product Line TSKgel
2

Thermo Scientific™ Acclaim™ PepMap™ C18 Peptide Analysis HPLC Column, 5 μm, 0.2 mm x 10 mm

Column Format: Pre-Column Traps; Carbon Load: 15%; Pore Size: 100 Å; Column Phase: Reversed Phase

3

MilliporeSigma™ Supelco™ Discovery™ HS F5 HPLC Column, 5 μm Particle Size

The Discovery™ HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase and HILIC
Matrix PFP (Pentafluorophenyl) Phase/Silica Gel, High Purity, Spherical Particle Platform
Endcapped Yes
pH 2 to 8
Surface Area 300 m2/g
Product Line Discovery
4

MilliporeSigma™ Supelco™ BIOshell™ IgG 1000 Å Diphenyl HPLC Column, 2.7 μm Particle Size

A high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core™ particle design.

Pore Size 1000 Å
Particle Size 2.7 μm
Carbon Load 1%
Stationary Phase Diphenyl (diphenylmethyl)
Phase Reversed Phase
Matrix Diphenyl (diphenylmethyl) Phase/Fused-Core™ Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Surface Area 22 m2/g
Product Line BIOshell
USP Type L11
5

MilliporeSigma™ Supelco™ SRT™-C SEC-100 Gel Filtration Column, 5 μm Particle Size, PEEK

The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

6

MilliporeSigma™ Supelco™ Antibodix™ WCX-NP10 Weak Cation Exchange Column, 10 μm Particle Size, PEEK

Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Spherical PS-DVB
Phase Cation Exchange
Matrix Spherical PS-DVB Phase
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Antibodix
7

Thermo Scientific™ Hypersil GOLD™ C4 HPLC Columns

Featuring a lower hydrophobicity than C18 or C8 columns, Thermo Scientific™ Hypersil GOLD™ C4 columns provide outstanding sensitivity and high efficiency.

Pore Size 175 Å
Carbon Load 5%
Stationary Phase C4
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
pH 2 to 8
Surface Area 220 m²/g
USP Type L26
8

MilliporeSigma™ Supelco™ Tenax™ GR Thermal Desorption Tubes

For use with Gerstel™ TDS A & TDS 2

Matrix Tenax™ GR Composite (30% Graphite Carbon, 70% Tenax™ TA)
Description Pre-conditioned
For Use With (Equipment) Gerstel™ TDS A & TDS 2
Diameter (Metric) Outer 6 mm
Diameter (Metric) Inner 4 mm
Packaging Quantity 1/Pk.
Overall Length (English) 7 in.
For Use With (Application) Air Monitoring
Product Line Tenax
9

MilliporeSigma™ Supelco™ Kromasil C18 HPLC Column, 100 Å, 3.5 μm Particle Size

For small molecules and peptides.

Pore Size 100 Å
Particle Size 3.5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Product Line Kromasil
10

TSKgel™ G5000HHR HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel G5000HHR Columns are packed with rigid, porous, polymer beads. They are from a class of columns used for the non-aqueous separation of polymers with molecular weights less than 4,000,000 Da.

11

Thermo Scientific™ POROS™ Heparin 50 μm Affinity Columns
GREENER_CHOICE

Thermo Scientific™ POROS™ Prepacked Heparin, Affinity Column. POROS Heparin is based on an immobilized Heparin functional group designed for high throughput purification of proteins or viruses with specific Heparin affinity.

Particle Size 50 μm
Stationary Phase Heparin (Porcine)
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 750 psi (50 bar)
Product Line POROS
12

Thermo Scientific™ POROS™ R1 Prepacked Reversed-Phase Columns

Thermo Scientific™ POROS™ Prepacked Reversed-Phase Column. POROS™ R1 is a 4000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.

Shipping Condition Room Temperature
Pore Size 4000 Å
Content And Storage Store at room temperature.
Quantity 1 Column
Product Line POROS
13

Thermo Scientific™ POROS™ HS 20 μm Strong Cation Exchange HPLC Columns
GREENER_CHOICE

Thermo Scientific™ POROS™ Prepacked Strong Cation Exchange Column. POROS HS is a high capacity, strong cation exchange, High Performance Chromatography™ resins suitable for the separation and purification of biomolecules.

Shipping Condition Room Temperature
Pore Size 2000 Å
Particle Size 20 μm
Type Prepacked Column
Content And Storage Store at room temperature.
Stationary Phase Sulfopropyl Polymer
Binding Capacity 75 mg/mL (Lysozyme pH 6.2)
Column Type Ion Exchange
Quantity 1 Column
Material PEEK
Product Line POROS
14

MilliporeSigma™ Supelco™ Carbotrap™ 217 Thermal Desorption Tubes

Suitable for use with Air Monitoring

Matrix Carbotrap™ 217, Carbotrap™ B Graphitized Carbon Black (GCB), Carboxen™ 1000 Carbon Molecular Sieve (CMS)
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Packaging Quantity 10/Pk.
Overall Length (English) 3.5 in.
For Use With (Application) Air Monitoring
Methods EPA TO-14, EPA TO-17
Product Line Carbotrap
15

MilliporeSigma BIOshell A160 Peptide PCS-C18 HPLC Column

BIOshell™ A160 Peptide PCS-C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins.

Pore Size 160 Å
Particle Size 2.7 μm
Type HPLC Column
Phase Positively Charged Surface (PCS)
Material Stainless Steel
Technique HPLC