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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 931 results
1

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
2

MilliporeSigma™ Supelco™ Proteomix™ SCX-NP10 Strong Cation Exchange Column, 10 μm Particle Size, PEEK

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Sulfonic Acid
Phase Cation Exchange
Matrix Spherical PS-DVB/Sulfonic Acid Phase
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Proteomix
3

MilliporeSigma™ Supelco™ CHIRALPAK™ CBH HPLC Column, 5 μm Particle Size

CHIRALPAK™ CBH phase has cellobiohydrolase as the chiral selector immobilized on spherical 5 μm silica particles.

Particle Size 5 μm
Stationary Phase Cellobiohydrolase
Phase Chiral
Matrix Cellobiohydrolase Phase
Technique Chiral HPLC
Product Line CHIRALPAK
4

Thermo Scientific™ Aquasil™ 3μm C18 HPLC Columns

Available in limited dimensions. Please contact Customer Service for more details.

Pore Size 100 Å
Particle Size 3 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
5

TSKgel™ GMHHR-H(S) HT HPLC Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel HT (High Temperature) Columns are for the analysis of organic-soluble polymers at temperatures up to 140°C. They are shipped in o-dichlorobenzene (ODCB), which can be exchanged for 1-chloronaphthalene and trichlorobenzene.

6

TSKgel™ Multipore HXL-M HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel MultiporeHxl-M Columns are packed with rigid, polymer beads that have a large pore size distribution within each particle. For the analysis of organic-soluble industrial polymers.

7

Thermo Scientific™ Hypersil™ CPS Cyano HPLC Columns

When you need a different selectivity in normal phase or a complementary selectivity to alkyl chains in reverse phase, use Thermo Scientific™ Hypersil™ CPS Cyano LC Columns.

Pore Size 120 Å
Carbon Load 4%
Stationary Phase Cyano (CN)
Temperature 60°C
Phase Normal and Reversed Phase
Endcapped No
Column Type Mixed Mode
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L10
8

TSKgel™ SuperQ-5PW HPLC Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel SuperQ-5PW Columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchanger. For the separation and analysis of proteins, oligonucleotides, and other biologically active molecules.

9

Thermo Scientific™ Hypersil™ APS-2 Amino HPLC Columns

Analyze sugars with this versatile amino propyl phase of Thermo Scientific™ Hypersil™ APS-2 Amino LC Columns.

Pore Size 120 Å
Carbon Load 2%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped No
Surface Area 170 m²/g
USP Type L8
10

Thermo Scientific™ Accucore™ Polar Selectivity Kits

Verify optimum selectivity of Thermo Scientific™ Accucore™ HPLC columns for polar analytes.

Pore Size 80 Å
Particle Size 2.6 μm
Stationary Phase aQ C18, HILIC, PFP
For Use With UHPLC
Endcapped Yes
Packing Material Ultrapure Silica
Diameter (Metric) 2.1 mm
Surface Area 130 m²/g
Particle Shape Solid Core
Column Format Analytical Column Kit
11

TSKgel™ Amide-80 HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

For the analysis of saccharides, glycans, oligosaccarides, peptides, and polar compounds from natural products.

Shipping Condition Shipped in 85% Acetonitrile, 15% Water
Pore Size 100 Å
Solvents 0-100% Organic Solvent
Stationary Phase Carbamoyl
Temperature 10°C to 80°C
Length (Metric) 30 cm
Material Stainless Steel Hardware
Surface Area 450 m2/g
Particle Size 10 μm
Matrix Carbamoyl Phase/Silica Particle Platform
pH 2 to 7.5
Technique HILIC
Product Line TSKgel
USP Type L68
12

MilliporeSigma™ Supelco™ SUPELCOSIL™ ABZ+Plus HPLC Column, 3 μm Particle Size

Offer both high deactivation and unique selectivity.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
13

MilliporeSigma™ Supelco™ Ascentis™ Phenyl HPLC Column, 5 μm Particle Size

The Ascentis™ Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase Phenyl
Phase Reversed Phase
Matrix Phenyl Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
14

MilliporeSigma™ Supelco™ Antibodix™ WCX-NP3 Weak Cation Exchange Column, PEEK, 3 μm Particle Size

Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.

Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Spherical PS-DVB
Phase Cation Exchange
Matrix Spherical PS-DVB Phase
Length (Metric) 5 cm
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Antibodix
15

MilliporeSigma™ Supelco™ Kromasil™ Eternity™ C18 HPLC Column, 100 Å, 5 μm Particle Size

Kromasil™ Eternity™ and Eternity™XT can be used in a wider pH window than regular silica-based HPLC materials, giving more flexibility than ever when running analytical applications or optimizing for large-scale applications.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Surface Area 330 m2/g
Product Line Kromasil Eternity