HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

1 – 15 of 996 results

MilliporeSigma™ Supelco™ Discovery™ C18 HPLC Column, 5 μm Particle Size

Use Discovery™ C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Pricing and Availability
Specifications

Pore Size	180 Å
Particle Size	5 μm
Stationary Phase	C18 (Octadecyl)
Phase	Reversed Phase
Matrix	C18 (Octadecyl) Phase/Silica Gel, High Purity, Spherical Particle Platform
Endcapped	Yes
pH	2 to 8
Material	Stainless Steel
Surface Area	200 m²/g
Product Line	Discovery

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 10 μm Particle Size

Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	10 μm
Stationary Phase	C5 (pentyl)
Phase	Reversed Phase
Matrix	C5 (pentyl) Phase/Silica Particle Platform
Endcapped	Yes
Technique	Wide Pore HPLC
Surface Area	100 m2/g
Product Line	Discovery

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	16%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 9
Surface Area	320 m²/g
USP Type	L1

TSKgel™ Butyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Butyl-NPR columns are not as hydrophobic, i.e., less retentive, than Phenyl-5PW and Ether-5PW HIC columns. They are the best choice for high speed separations and provide excellent recovery even for more hydrophobic proteins.

Pricing and Availability

MilliporeSigma Ascentis™ Express PCS-C18 HPLC Column

Ascentis™ Express PCS-C18 columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.

Pricing and Availability
Specifications

Pore Size	90 Å
Particle Size	2.7 μm
Type	HPLC Column
Phase	Positively Charged Surface (PCS)
Material	Stainless Steel
Technique	HPLC

Thermo Scientific™ Acclaim™ C30 HPLC Columns

Use for superior separation of hydrophobic structural isomers.

Pricing and Availability
Specifications

Pore Size	200 Å
Carbon Load	13%
Stationary Phase	C30
Temperature	60°C
Phase	Reversed Phase
Column Type	Reversed Phase
pH	2 to 8
Surface Area	200 m²/g
Product Line	Acclaim

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	10 μm
Stationary Phase	C18 (octadecyl)
Phase	Reversed Phase
Matrix	C18 (octadecyl) Phase/silica particle platform
Endcapped	Yes
Technique	Wide Pore HPLC
Surface Area	100 m2/g
Product Line	Discovery

Thermo Scientific™ Accucore™ 150-C4 HPLC Columns

Use this specially designed solid core column with a C4 functionality for biomolecules that bind too tightly to C8 or C18 columns.

Pricing and Availability
Specifications

Pore Size	150 Å
Particle Size	2.6 μm
Carbon Load	2%
Stationary Phase	C4
Temperature	60°C
Phase	Reversed Phase
For Use With	HPLC
Endcapped	Yes
Packing Material	Spherical, Solid Core, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	80 m²/g
USP Type	L26

Thermo Scientific™ Accucore™ Phenyl-Hexyl HPLC Columns

Use this solid core column for fast, high-resolution separations of aromatic and moderately polar compounds.

Pricing and Availability
Specifications

Pore Size	80 Å
Temperature	70°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	130 m²/g
Max. Pressure	14500 psi (1000 bar)
Particle Size	2.6 μm
Carbon Load	5%
Packing Material	Spherical, Solid Core, Ultrapure Silica
pH	2 to 8
Particle Shape	Spherical Solid Core
Product Line	Accucore
USP Type	L11

Restek™ Pinnacle™ DB C8 Specialty/Application Specific HPLC Column, 5 μm

Base-deactivated spherical silica optimized for UHPLC stability.

Pricing and Availability
Specifications

Pore Size	140 Å
Stationary Phase	C8
Temperature	80°C
Phase	Reversed Phase
For Use With	Analysis of Basic Compounds or Bases Mixed with Acids/Neutrals
Endcapped	Yes
Column Type	Specialty/Application Specific
Quantity	5 g
Material	Stainless Steel
Max. Pressure	4000 psi (275 bar)
Particle Size	5 μm
Ligand Type	Monomeric C8
Carbon Load	6%
Packing Material	Silica
pH	2.5 to 10
Technique	UHPLC
Particle Shape	Spherical
Film Thickness	5 μm
USP Type	L7

Restek™ Viva™ HPLC Columns with C18 Column Packing, 5μm Particle Size

Wide bore silica columns excellent for general-purpose applications and separating peptides or proteins

Pricing and Availability
Specifications

Pore Size	300 Å
Stationary Phase	C18
Temperature	80°C
Phase	Reversed Phase
For Use With	Analyzing Large Molecules and Biomolecules
Endcapped	Yes
Column Type	Reversed Phase
Particle Size	5 μm
Type	Column
Carbon Load	9%
Packing Material	Silica
pH	2.5 to 10
Particle Shape	Spherical
Film Thickness	5 μm
USP Type	L1

MilliporeSigma™ LiChroCART™ Column/LiChrospher™ 100 RP-18 Endcapped Sorbent HPLC Cartridges, 5μm

HPLC cartridge

Pricing and Availability
Specifications

Pore Size	100 Å
Type	Cartridge
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Silica
Column Type	Reversed Phase
Material	Stainless Steel
USP Type	L1

Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: PEEK

Stable alternative to C8 or C18 Columns

Pricing and Availability
Specifications

Pore Size	100 Å
Stationary Phase	PRP-1
Temperature	5°C to 85°C
Phase	Reversed Phase
Column Type	Reversed Phase
Material	PEEK
Max. Pressure	5000 psi (345 bar)
Column Format	Analytical Column
Particle Size	10 μm
Flow Rate	1.0 mL/min
Type	Column
Packing Material	PSDVB (Polystyrene Divinylbenzene)
Mobile Phase Compatibility	pH 1 to 13, 0 to 100% aqueous, organic modifier
Diameter (Metric)	4.6 mm
pH	1 to 13
USP Type	L21

Hamilton™ PRP™-3 Reversed Phase HPLC Columns

PRP-3 polymeric 300 Å columns for gradient separations of peptides and proteins

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	10 μm
Stationary Phase	PRP-3
Temperature	5°C to 85°C
Phase	Reversed Phase
Packing Material	PSDVB (Polystyrene Divinylbenzene)
Column Type	Reversed Phase
pH	1 to 13
Max. Pressure	5000 psi (345 bar)
USP Type	L21

Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: Stainless Steel

Stable alternative to C8 or C18 Columns

Pricing and Availability
Specifications

Pore Size	100 Å
Type	Column
Stationary Phase	PRP-1
Temperature	5°C to 85°C
Phase	Reversed Phase
Packing Material	PSDVB (Polystyrene Divinylbenzene)
Column Type	Reversed Phase
Mobile Phase Compatibility	pH 1 to 13, 0 to 100% aqueous, organic modifier
pH	1 to 13
Material	Stainless Steel
Max. Pressure	5000 psi (345 bar)
USP Type	L21
Column Format	Analytical Column

HPLC Columns

Filtered Search Results

Narrow Results