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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,957)
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1 – 15 of 1,263 results
1

Thermo Scientific™ μPAC™ HPLC Columns

Learn more about your proteome, metabolome, and lipidome samples with Thermo Scientific™ μPAC™ HPLC columns.

Pore Size 100 to 200 Å
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Silicon Chip
Column Type Micro Pillar Array
pH 1.5 to 7
Interpillar Distance 2.5 μm
Porosity 59%
Pillar Diameter 5 μm
Max. Pressure 350 bar (5076 psi)
2

MilliporeSigma™ Supelco™ Ascentis™ Express OH5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express OH5 columns can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Hydroxy, Penta-
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
3

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 3 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
4

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase
Matrix PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 1 to 9
Technique Fused-Core HPLC
Product Line Ascentis
5

Thermo Scientific™ MAbPac™ HIC-Butyl HPLC Columns
GREENER_CHOICE

Separate monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) with excellent biocompatibility, low carryover and high resolution.

Pore Size Nonporous
Particle Size 5 μm
Temperature 60°C
Phase Hydrophobic Interaction
Packing Material Hydrophilic Polymer
Column Type Hydrophobic Interaction
pH 2 to 12
Quantity Each
Green Features FSC
Max. Pressure 4000 psi (275 bar)
Product Line MAbPac
6

Thermo Scientific™ Hypersil GOLD™ Cyano HPLC Columns

Alternate selectivity with lower hydrophobicity. Use these HPLC columns for normal and reversed phase separations.

Pore Size 175 Å
Carbon Load 4%
Stationary Phase Cyano (CN)
Temperature 60°C
Phase Normal and Reversed Phase
Endcapped Yes
pH 2 to 8
Surface Area 220 m²/g
USP Type L10
7

Thermo Scientific™ Acclaim™ Trinity P1 HPLC Columns
GREENER_CHOICE

Use these mixed-mode columns for simultaneous separation of pharmaceutical drug substances and their counter ions with high efficiency.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase Reversed Phase/Anion Exchange/Cation Exchange
Temperature 45°C
Phase Reversed Phase and Ion Exchange
Endcapped Proprietary
Column Type Mixed Mode
pH 2.5 to 7
Green Features FSC
Surface Area 100 m²/g
Max. Pressure 4500 psi (310 bar)
Product Line Acclaim
8

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns
GREENER_CHOICE

Use these wide-pore C18 columns to achieve high-resolution protein separations.

Pore Size 300 Å
Stationary Phase C18
Temperature 60°C
For Use With LC/MS
Endcapped Yes
Column Type Reversed Phase
Green Features FSC
Surface Area 100 m²/g
Particle Size 3 μm
Flow Rate 1 mL/min
Carbon Load 8%
pH 2.5 to 7.5
Product Line Acclaim
USP Type L1
9

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
12

MilliporeSigma™ Supelco™ GreenSep™ DEAP SFC HPLC Column, 5 μm Particle Size

Unlike many SFC columns which require the addition of amine modifiers to the mobile phase to improve peak shape for basic compounds, Greensep™ DEAP does not, greatly simplifying mobile phase composition and fraction collection.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase DEAP
Phase SFC
Matrix DEAP Phase
Technique SFC HPLC
Product Line GreenSep
13

Thermo Scientific™ ProPac™ SCX-20 Strong Cation Exchange HPLC Column
GREENER_CHOICE

Meet today's demands in protein separations using this high resolution strong cation exchange (SCX) HPLC column.

Stationary Phase Sulfonate
Temperature 60°C
Phase Ion Exchange
Column Type Ion Exchange
Quantity Each
Green Features FSC
Max. Pressure 3000 psi (207 bar)
Column Format Analytical Column
Particle Size 10 μm
Packing Material Ethylvinylbenzene Divinylbenzene
Diameter (Metric) 4 mm
pH 2 to 12
Technique Bio-LC
Product Line ProPac
14

MilliporeSigma™ Supelco™ Carbosieve™ SIII Sampling Tube, Prepacked

Thermally Conditioned and Batch Tested for Backpressure and Background

15

MilliporeSigma™ Supelco™ Graphsphere™ 2016 Glass Thermal Desorption Tubes

Fritted and Preconditioned TD Tubes

Type Thermal Desorption Tube
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Material Glass
Overall Length (English) 3.5 in.
For Use With (Application) An Alternative to Tenax™ TA