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Invitrogen™ pBAD TOPO™ TA Expression Kit 
Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products
Supplier: Invitrogen™ K430040
Description
- Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
- araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
- C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
- Enterokinase cleavage site for removal of N-terminal leader peptide
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Spécifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| EK (Enterokinase) Recognition Site | |
| Cell-Based Expression | |
| TOPO-TA | |
| pBAD |
| Arabinose | |
| TA Expression Kit | |
| Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194 E. coli stab at at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| LMG194, TOP10 | |
| E. coli | |
| V5 Epitope Tag | |
| 40 Reactions | |
| TOPO |
For Research Use Only. Not for use in diagnostic procedures.