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Invitrogen™ pBAD TOPO™ TA Expression Kit DFS Item

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

$1,308.00 - $2,200.00


Constitutive or Inducible System Inducible
Promoter araBAD
Inducing Agent Arabinose
Selection Agent (Eukaryotic) None
Vector pBAD
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For Research Use Only. Not for use in diagnostic procedures.

Includes: Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Protein Tag Price Quantity  
Catalog Number Mfr. No. Quantity Protein Tag Price Quantity  
K430040 Invitrogen™
40 reactions Each for $2,200.00
K430001 Invitrogen™
20 reactions Each for $1,308.00


  • Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
  • araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
  • C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Description & Specifications