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Invitrogen™ pBAD TOPO™ TA Expression Kit DFS Item

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

$1,253.70 - $2,131.50

Specifications

Constitutive or Inducible System Inducible
Promoter araBAD
Inducing Agent Arabinose
Selection Agent (Eukaryotic) None
Vector pBAD
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Includes:  Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

K430040

DFS Item
 
invitrogen™
K430040
40 reactions Each for $2,131.50

K430001

DFS Item
 
invitrogen™
K430001
20 reactions Each for $1,253.70
Description & Specifications

Specifications

Constitutive or Inducible System Inducible
Promoter araBAD
Inducing Agent Arabinose
Selection Agent (Eukaryotic) None
Vector pBAD

  • Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
  • araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
  • C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis