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Thermo Scientific™ EZ-Link™ NHS-SS-PEG4-Biotin
Biotinylate protein amines with this cleavable, long (37.9A) NHS-ester biotinylation reagent containing a reducible disulfide bond in the pegylated, water-soluble spacer arm.
Supplier: Thermo Scientific™ 21442
Description
Thermo Scientific™ EZ-Link NHS-SS-PEG4-Biotin includes disulfide and polyethylene glycol (PEG) groups in its spacer arm to provide biotinylation that is cleavable and which enhances solubility.
Highlights:
- Extended spacer arm reduces steric hindrance in binding to avidin, streptavidin and Thermo Scientific NeutrAvidin Protein
- PEG spacer maintains water solubility of the modified compound
- Disulfide bond in the spacer arm enables the biotin group to be cleaved from the protein by treatment with reducing agent
- Useful in affinity purification experiments where it is necessary to recover biotin labeled protein from immobilized avidin, streptavidin or NeutrAvidin™ Supports
Spécifications
| NHS-SS-PEG4-Biotin | |
| Store desiccated at -20°C. Shipped at ambient temperature. | |
| Cell-Permeant | |
| Active Ester, Succinimidyl Ester, NHS Ester | |
| Biotin | |
| Biotin & Analogs |
| 50 mg | |
| EZ-Link | |
| Amine | |
| DMF (Dimethylformamide), DMSO (Dimethylsulfoxide) | |
| Extra-long, Pegylated, Cleavable |
For Research Use Only. Not for use in diagnostic procedures.