Learn More About QuantStudio Real-Time PCR & Digital PCR Systems
Every lab is unique. That’s why you deserve a real-time PCR or digital PCR platform that fits your needs. Perhaps you’re looking for simplicity on a budget, or reliable results from limited samples. Maybe your research requires high throughput for maximum productivity, or absolute answers to take your work to the next level. Whatever you need, there’s an Applied Biosystems™ QuantStudio™ Real-Time PCR or Digital PCR System that’s just right for your research.
Visit fishersci.ca/qpcrreagents to find qPCR reagents to use with your QuantStudio System.
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QuantStudio™ Real-Time PCR Systems Comparison
| Feature | QuantStudio™ 3 | QuantStudio™ 5 | QuantStudio™ 6 Pro | QuantStudio™ 7 Pro | QuantStudio™ 12K Flex |
|---|---|---|---|---|---|
| Block format | Fixed block | Fixed block | Fixed block (Upgradeable to QS7) | Interchangeable block | Interchangeable block |
| Blocks | 96-well (0.1 mL or 0.2 mL) | 96-well (0.1 mL or 0.2 mL); 384-well | 96-well (0.1 mL or 0.2 mL); 384-well | 96-well; 384-well; TaqMan™ Array Card | 96-well; 384-well; TaqMan™ Array Card; OpenArray™ |
| Multiplexing | Up to 4 targets | 96-well: up to 6 targets; 384-well: up to 5 targets | Up to 5 targets | Up to 6 targets (96- and 384-well) | 96-/384-well: up to 6 targets; TaqMan™ Array Card: up to 3; OpenArray™: up to 2 |
| Automation-ready | No | No | Upgradeable to QS7 | Yes | Yes |
| Run time | < 30 min runs | 96-well: < 30 min; 384-well: < 35 min | 96-well: < 30 min; 384-well: < 35 min | 96-well: < 30 min; 384-well: < 35 min | 96-well: < 30 min; 384-well: < 35 min |
| VeriFlex™ block technology | Yes | Yes | Yes | Yes | No |
| Gradient capacity | VeriFlex™ 3 Zone (5°C zone-to-zone) | VeriFlex™ 6 Zone (5°C zone-to-zone) | VeriFlex™ 3 Zone (5°C zone-to-zone) | VeriFlex™ 6 Zone (5°C zone-to-zone) | N/A |
| Filter/colors | 4 coupled excitation/emission filters | 6 decoupled excitation/emission filters (0.1 or 0.2 mL) | 5 coupled excitation/emission filters | 6 decoupled excitation/emission filters (21 filter combos) | 6 coupled excitation/emission filters (21 filter combos) |
| Dye compatibility* | FAM™/SYBR™ Green; VIC™/JOE™/HEX™/TET™; ABY™/NED™/TAMRA™/Cy®3; JUN™/ROX™/Texas Red™; custom dyes | Same as QS3 plus Mustang Purple™, Cy®5, LIZ™, Alexa Fluor™ 647, Cy®5.5 (96-well only); custom dyes | Same as QS5 (without Alexa Fluor™ 647 note); custom dyes | Same as QS6 Pro; custom dyes | Same as QS7 Pro; custom dyes |
| 21 CFR Part 11 support | No | Yes | No | Yes | Yes |
| Footprint (W × D × H) | 27 × 50 × 40 cm | 27 × 50 × 40 cm | 33.8 × 52.5 × 54.7 cm | 33.8 × 52.5 × 54.7 cm | 53 × 70 × 75 cm |
| Touchscreen | 8.4-inch capacitive | 8.4-inch capacitive | 12-inch capacitive | 12-inch capacitive | 10.4-inch capacitive |
| System configuration | Stand-alone, PC connected, or LAN/Wi-Fi via Connect Platform | Stand-alone, PC connected, or LAN/Wi-Fi via Connect Platform | Stand-alone, PC connected, or LAN/Wi-Fi via Connect Platform | Stand-alone, PC connected, or LAN/Wi-Fi via Connect Platform | PC connected, LAN |
| Dynamic range | 10 logs | 10 logs | 10 logs | 10 logs | 10 logs (OpenArray™: 7 logs) |
| Analytical sensitivity | 1 copy | 1 copy | 1 copy | 1 copy | 1 copy |
| Discrimination / resolution | 1.5-fold concentrations | 1.5-fold concentrations | 1.5-fold concentrations | 1.5-fold concentrations | 1.5-fold (OpenArray™: 2-fold) |