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Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System, 96-well, 0.1 mL

Catalog No. a28136
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Catalog No. A28136 Supplier Applied Biosystems™ Supplier No. A28136

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

Computer not included

QuantStudio™ 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality.

The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio™ 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex™ technology (featuring 4 coupled channels and white LED) and featuring three independent VeriFlex™ temperature zones, the QuantStudio™ 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

Features of the QuantStudio™ 3 system include:
  • Simplified instrument software with interactive touchscreen for greater ease of use
  • Proven OptiFlex™ technology (4 coupled channels with white LED) for trusted results
  • Secure cloud-based software option provides increased accessibility and storage options
  • User account management, locked workflow, and Pause features for greater control of experiments
  • 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud:
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use:
The QuantStudio™ 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio™ Design and Analysis cloud software
  • Supports web browser-based system configuration with PC or MAC computer
  • Streamlined software for improved usability and response time
  • Enables secure access of your data when and where you want through the cloud
  • No software to install, no licenses and no versions to update

Option 2: QuantStudio™ Design and Analysis desktop software
  • Supports traditional co-located computer system configuration on Windows™ 7 OS
  • Streamlined software for improved usability and response time
  • No licenses to purchase

Results you can trust:
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex™ technology, which features 4 coupled excitation and emission filter channels with white LED.

Sensitive data analysis:
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility:
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

For Research Use Only. Not for use in diagnostic procedures.

Order Info

Room Temperature

Specifications

Block Format Non-interchangeable
Format 96-well Plate
Content And Storage Computer not Included
For Use With (Equipment) QuantStudio™
Product Line QuantStudio
Display Type Touchscreen
Shipping Condition Room Temperature
Volume (Metric) Thermal Block Sample 0.1 ml
Quantity 1 System

Frequently Asked Questions (FAQs)

How should I program my QuantStudio 3 Real-Time PCR System, 96-well, 0.1 mL (Cat. No. A28136) to see data with a RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) kit?

The exact run method and temperatures will be dependent on the particular kit, but it will be important to program a 2-step PCR stage so that sufficient data points can be collected. For example, for a 40 min incubation at 65 degrees C, can be set up as the following:
- PCR, 40 cycles
- Step 1, 65C, 5 sec
- Step 2, 65C, 55 sec (data collection ON)
Times and number of cycles can be adjusted as needed to collect more data points.

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.
Can I downgrade the firmware on my QuantStudio 5?

You can perform this action if you have access to an Admin account on the QuantStudio instrument and if it is compatible for the downgrade (newer instruments are not compatible with v1.3.3). If your instrument serial number is below the following, then it can be downgraded:
QuantStudio 5 96-well, 0.1 mL block - SN 272514140
QuantStudio 5 96-well, 0.2 mL block - SN 2725210507
QuantStudio 5 384 well block - SN 272533285
Please also check if the instrument is being used with any validated protocols that may require a particular firmware version to be compliant. If you need the firmware itself, you can download it here.
The instructions to downgrade the firmware are on p.4 of the Release Notes: QuantStudioTM 3 & 5 Touchscreen Instrument Operating Software Version 1.3.3. It's the same process as an upgrade.
The calibration on my real-time PCR instrument has expired and I get an error message to calibrate my machine. Is it possible to turn off the reminders and carry on with my experiments without calibrating the machine?

Yes, you can click okay to the message and continue with the run and calibrate the instrument at a later time. The exception is that the QuantStudio Dx, QuantStudio 5 Dx, and QuantStudio 7 Pro Dx instruments cannot be run in IVD mode with expired calibrations.
What is the admin pin for QuantStudio 3 and 5 Real-Time PCR Systems?

If the admin PIN has not been changed, 1234 should work. If it does not work, you can also try 0000.
However, if the admin PIN has been changed and none of the above PINs work, follow the instructions in the QuantStudio 3 and 5 Real-Time PCR Systems manual, page 100.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Design & Analysis Software (v1.x)?

If you are analyzing results from the QuantStudio 1 Real-Time PCR System, QuantStudio 3 Real-Time PCR System, or QuantStudio 5 Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Design & Analysis Software (v1.x) following the instructions below:
1. Open the data file in the QuantStudio Design & Analysis Software (v1.x).
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply.
Can I correct errors in sample and target names on the QuantStudio 3 Real-Time PCR System (Cat. No. A28136), after the run has been completed?

Yes. On all of our QuantStudio instruments, the sample names, target names, and other plate layout assignments can be adjusted after the run. The instrument always collects all the data in all filter channels during the run, and uses the plate setup information to know how to analyze the data. When changing the plate layout after a run has been completed, simply make the desired changes and press the "Analyze" button to apply the changes.

Please note that changes to the Experiment Type (outside of shifting between "Standard Curve", "Relative Standard Curve", and "Comparative Ct") and changes to the run method are not possible after a run has completed.
Can you provide some tips for setting up an absolute quantification/standard curve on the QuantStudio 3 or 5 Real-Time PCR instrument?

Please review this guided tutorial that explains how to set up an Absolute Quantification/Standard Curve experiment run on your QuantStudio 3 or 5 Real-Time PCR instrument: https://www.brainshark.com/thermofisher/QS35AQSetupTutorial
How do I set up the QuantStudio 3 or QuantStudio 5 Real-Time PCR System for WiFi access?

To set up the QuantStudio 3 or QuantStudio 5 Real-Time PCR System for WiFi capability, we recommend following the instructions in the manual (https://tools.thermofisher.com/content/sfs/manuals/MAN0010407_QuantStudio3_5_InstallUseMaint_UG.pdf). You will also need to purchase the High Power USB WiFi Module (Cat. No. A26774).

Can I use templates from other instruments to start a run in the QuantStudio 3 or QuantStudio 5 Real-Time PCR System?

Yes, you can use templates from other compatible systems, if you use the Thermo Fisher Cloud Design and Analysis module. Use the function “New Experiment --> From File” to import the .edt file.
I am having trouble connecting to my Thermo Fisher Cloud account, what could be wrong?

Check if the Cloud Monitor is on. Go to Settings, then Maintenance and Service, then Monitoring. Turn CloudSuite Monitor ‘ON'. This needs to be set to ‘ON', otherwise the instrument will not look out to connect to your account even if you are logged in.
Are my run files from the QuantStudio 3 or 5 Real-Time PCR System compatible with TaqMan Genotyper Software?

No. You can use the QuantStudio Design and Analysis Desktop Software, or for multi-plate analysis of genotyping data, please use the Applied Biosystems qPCR Analysis Module (GT application) with your Thermo Fisher Cloud account.
Are my run files from the QuantStudio 3 or 5 Real-Time PCR System compatible with ExpressionSuite Software?

Yes, ExpressionSuite Software v1.1 is compatible with files from the QuantStudio 3 and 5 Real-Time PCR Systems..
I have a qPCR master mix from another vendor that has ROX passive reference dye in a separate tube. How much should I add for my reactions in the QuantStudio 3 and 5 Real Time PCR Systems?

If you are using a master mix that has ROX provided in a separate tube, use a low ROX concentration (50 nM, final well volume).
How do I analyze multiple ddCt files together from a QuantStudio 3 or 5 Real-Time PCR System?

Use the Applied Biosystems qPCR Analysis Module (RQ application) with your Thermo Fisher Cloud account.
How do I start a qPCR run on the QuantStudio 3 or 5 Real-Time PCR System using the cloud?

1. Set up the run in the cloud from the Design and Analysis application within the Applied Biosystems qPCR Analysis Modules.
2. Save the experiment before starting a run: The file name was created from the experiment Name entered in the Properties tab. However, changes to the experiment Name in the Properties tab after the first save do not update the file name. To change the file name before a run, select Actions, then Save As.
3. Go to an available cloud-connected instrument, enter your PIN to access this file, and start the run by choosing ‘Open Template'. Following a run, view experiment details, run duration, and instrument and software information in the Run Control screen of the Run tab.
Can I start a run on the QuantStudio 3 or 5 Real-Time PCR System from the Thermo Fisher Cloud Design and Analysis application?

You can set up your run in the Design and Analysis application, but to actually start the run, you will have to go to the instrument touchscreen.
Can I monitor my qPCR run from the QuantStudio 3 or 5 Real-Time PCR System in real time?

Yes, you have several options for this. You can monitor the run in real time from:

- The instrument touchscreen
- The QuantStudio Design and Analysis Desktop Software
- Online using the Design and Analysis application within Thermo Fisher Connect
- Online using the Instrument Connect App on your Android or Apple mobile device - for more on this, please see here (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/quantstudio-3-5-real-time-pcr-system/connectivity-quantstudio-3-5-qpcr-data-analysis-software.html).

With the QuantStudio 3 or QuantStudio 5 Real-Time PCR System, I want my file to go to my Thermo Fisher Cloud account once the run is complete for analysis. Is there a way for it to automatically upload?

Yes. To have the run file automatically upload to your Thermo Fisher Cloud account once complete, you will need to set the file destination on the touchscreen, prior to starting the run:
1. Before you start the run, select the ‘Properties' tab from an open experiment
2. Touch ‘Edit', Data Destination - Choose USB or Cloud
Alternatively, you could use Cloud Connect Utility to have all files from a specific hard drive folder location automatically upload to the cloud (for use with the QuantStudio Design and Analysis Desktop Software).
How do I open the drawer in the QuantStudio 3 or QuantStudio 5 Real-Time PCR System?

The block drawer is opened and closed by using the ‘eject' button on the touchscreen. Do not push or pull on the instrument drawer.
When do I have to calibrate the QuantStudio 3 and QuantStudio 5 Real Time PCR Systems?

When your new QuantStudio 3 or QuantStudio 5 Real-Time PCR System arrives in your lab, it will be factory calibrated. The recommended calibration schedule is to recalibrate every 2 years.
Can I purchase or use the QuantStudio 3 or QuantStudio 5 Real Time PCR System without a computer?

Yes, you can purchase the QuantStudio 3 or QuantStudio 5 Real Time PCR System without a computer. In this configuration, you can run the instrument through the touchscreen or the Design and Analysis application from Thermo Fisher Cloud.
Do the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems have the ability for security and auditing software?

Yes, the QuantStudio 5 Real-Time PCR System has SAE (Security/Auditing/e-Signature) features that can be enabled in the QuantStudio Design and Analysis Desktop Software.
What is the difference between the Applied Biosystems QuantStudio 3 and Applied Biosystems QuantStudio 5 Real-Time PCR Systems?

The QuantStudio 3 Real-Time PCR System is a 4-color instrument with a 3-zone VeriFlex block, while the QuantStudio 5 Real-Time PCR System is a 6-color instrument with 6-VeriFlex zones in a 96-well block, (or 5-color format, no VeriFlex with 384-well block).

For Research Use Only. Not for use in diagnostic procedures.