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Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, 96-well, 0.1 mL

Catalog No. a28138
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Catalog No. A28138 Supplier Applied Biosystems™ Supplier No. A28138

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

No Computer

Designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use.

The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio™ 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex™ technology (featuring 6 decoupled channels and white LED) and featuring six independent VeriFlex™ temperature zones, the QuantStudio™ 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio™ 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Features of the QuantStudio™ 5 system with 96-well block include:

  • Proven OptiFlex™ technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
  • User account management, locked workflow, and Pause features for greater control of experiment data
  • Built-in software features to support compliance with 21 CFR Part 11 guidelines
  • Secure cloud-based software provides increased accessibility and storage options
  • Simplified instrument software with interactive touchscreen for greater ease of use
  • 96-well 0.2mL format or 96-well 0.1mL format

Thermo Fisher Cloud:
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

Easy to use:
The QuantStudio™ 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio™ Design and Analysis cloud software

  • Supports web browser-based system configuration with PC or MAC computer
  • Streamlined software for improved usability and response time
  • Enables secure access of your data when and where you want through the cloud
  • No software to install, no licenses and no versions to update

Option 2: QuantStudio™ Design and Analysis desktop software

  • Supports traditional co-located computer system configuration on Windows™ 7 OS
  • Streamlined software for improved usability and response time
  • No licenses to purchase

Total control:
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio™ 5 system offers users greater control of experiment data

Multiplex performance results that you can trust:
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex™ technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis:
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility:
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

For Research Use Only. Not for use in diagnostic procedures.

Order Info

Room Temperature

Specifications

Block Format Non-interchangeable
Format 96-well Plate
For Use With (Equipment) QuantStudio™
Product Line QuantStudio
Display Type Touchscreen
Shipping Condition Room Temperature
Volume (Metric) Thermal Block Sample 0.1 ml
Quantity 1 system

Frequently Asked Questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.
The calibration on my real-time PCR instrument has expired and I get an error message to calibrate my machine. Is it possible to turn off the reminders and carry on with my experiments without calibrating the machine?

Yes, you can click okay to the message and continue with the run and calibrate the instrument at a later time. The exception is that the QuantStudio Dx, QuantStudio 5 Dx, and QuantStudio 7 Pro Dx instruments cannot be run in IVD mode with expired calibrations.
What is the admin pin for QuantStudio 3 and 5 Real-Time PCR Systems?

If the admin PIN has not been changed, 1234 should work. If it does not work, you can also try 0000.
However, if the admin PIN has been changed and none of the above PINs work, follow the instructions in the QuantStudio 3 and 5 Real-Time PCR Systems manual, page 100.

How do I download the Pathogen Interpretive Software?

Once you place your order, please contact our Instrument Service team at 1-800-955-6288 option 3, 1 (this kit is only available in North America). You will be given an access code to the TaqPath Monkeypox/Orthopox Virus DNA Kit eLearning where you’ll learn how to install, update, and download the Pathogen Interpretive Software package for the TaqPath Monkeypox/Orthopox Virus DNA Kit.
With the TaqPath Monkeypox/Orthopox Virus DNA Kit, what software should I use for data analysis?

Results are analyzed using the following software:
- Applied Biosystems Pathogen Interpretive Software v1.1
- SAE Administrator Console Dx v1.0 or v1.2 (for security and audit functions)
- Pathogen Interpretive Software 1.0.0 DAT (SAE Profile)

Please use the appropriate assay panel and template for your instrument:
- MPXOPX-EUA_QS5-9601_1.0.0.zip
- MPXOPX-EUA_QS5Dx-9602_1.0.0.zip
- MPXOPX-EUA_QS7F-384_1.0.0.zip

Note: These zip files are located in EducationConnect (www.thermofisher.com/educationconnect). The eLearning course needs to be completed to be granted access.
What is the instrument software configuration required to use the TaqPath Monkeypox/Orthopox Virus DNA Kit?

To obtain the correct analysis software or firmware version for your real-time PCR instrument, go to thermofisher.com/validatedfirmware, then select your instrument in the Real-Time PCR section.
Which instruments are authorized under the EUA for use with the TaqPath Monkeypox/Orthopox Virus DNA Kit?

Here are the instruments that can be used with the TaqPath Monkeypox/Orthopox Virus DNA Kit:
- Applied Biosystems QuantStudio 5 Real-Time PCR System (96-well, 0.1 mL block)
- Applied Biosystems QuantStudio 5 Dx Real-Time PCR System (96-well, 0.2 mL block)
- Applied Biosystems QuantStudio 7 Flex Real-Time PCR System (384-well block)
I would like to use the TaqPath Monkeypox/Orthopox Virus DNA Kit. Which extraction kit should I use to extract DNA from the sample?

Performance was established using the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (Cat. No. A48383) in combination with the KingFisher Flex Magnetic Particle Processor with 96 Deep-Well Head and the KingFisher Flex 96 Deep-Well Heating Block (MVP_2Wash_400_Flex program). Other purification methods may lead to deviations in the expected result.
Which specimen types are compatible with the TaqPath Monkeypox/Orthopox Virus DNA Kit?

The TaqPath Monkeypox/Orthopox Virus DNA Kit protocol is currently authorized for use with lesion swab specimens in universal transport media (UTM) or viral transport media (VTM) from individuals suspected of monkeypox infection by their healthcare provider.
What instruments, consumables, and reagents do I need for the TaqPath Monkeypox/Orthopox Virus DNA Kit? Where can I find the full list of EUA products and protocols?

Go to www.thermofisher.com/monkeypox and download the Instructions for Use (IFU) to see the workflow outlining the instruments, consumables, and reagents required to run Monkeypox detection experiments.
What are the reporter dyes and targets for the TaqPath Monkeypox/Orthopox Virus DNA Kit?

The reporter dyes used are as follows:
- Monkeypox virus: FAM dye
- Orthopoxvirus: VIC dye
- RNase P: JUN dye

The TaqPath Monkeypox/Orthopox Virus DNA Kit probes contain QSY and MGB quenchers, which do not fluoresce. Select None for Quencher in the instrument set up procedure.
What are the components of the TaqPath Monkeypox/Orthopox Virus DNA Kit? Is the master mix included, or does it need to be purchased separately? Are there any other required materials that are not supplied?

The TaqPath Monkeypox/Orthopox Virus DNA Kit includes the following components:
- TaqPath Monkeypox/Orthopox Virus Multiplex Assay: Multiplexed real-time PCR assay containing primers/probes specific to monkeypox virus, non-variola orthopoxvirus targets, and human RNase P.
- TaqPath Monkeypox/Orthopox Virus DNA Positive Control: Positive DNA control that contains templates specific to monkeypox virus, non-variola orthopoxvirus targets, and human RNase P regions targeted by the assay.

Note:
- The master mix is not included in the kit and needs to be purchased separately: TaqPath BactoPure Microbial Detection Master Mix (No ROX) (Cat. No. A52703, A52704, or A52705)
- TE Buffer (Cat. No. 12090015) and Nuclease-free water (MLS) are required and not supplied with the kit.
Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Design & Analysis Software (v1.x)?

If you are analyzing results from the QuantStudio 1 Real-Time PCR System, QuantStudio 3 Real-Time PCR System, or QuantStudio 5 Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Design & Analysis Software (v1.x) following the instructions below:
1. Open the data file in the QuantStudio Design & Analysis Software (v1.x).
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply.
Can you provide some tips for setting up an absolute quantification/standard curve on the QuantStudio 3 or 5 Real-Time PCR instrument?

Please review this guided tutorial that explains how to set up an Absolute Quantification/Standard Curve experiment run on your QuantStudio 3 or 5 Real-Time PCR instrument: https://www.brainshark.com/thermofisher/QS35AQSetupTutorial
I am interested in running a multiplex qPCR assay on the QuantStudio 5 Real-Time PCR System, using ROX dye as the passive reference dye. Which reporter dyes are not compatible with ROX as the passive reference dye?

Texas Red and JUN dyes are not compatible with the ROX dye as they are read by the same channel in the instrument.
How do I analyze an SDS/EDS run if I am using probes with non-Applied Biosystems quenchers such as Black Hole Quencher (BHQ), ZEN, or Iowa Black RQ in the QuantStudio software?

To set up probes with non-Applied Biosystems quenchers, simply change the Quencher option to “NONE” under the “Advanced setup” tab in the Plate section of the QuantStudio software.

How do I set up the QuantStudio 3 or QuantStudio 5 Real-Time PCR System for WiFi access?

To set up the QuantStudio 3 or QuantStudio 5 Real-Time PCR System for WiFi capability, we recommend following the instructions in the manual (https://tools.thermofisher.com/content/sfs/manuals/MAN0010407_QuantStudio3_5_InstallUseMaint_UG.pdf). You will also need to purchase the High Power USB WiFi Module (Cat. No. A26774).

Can I use templates from other instruments to start a run in the QuantStudio 3 or QuantStudio 5 Real-Time PCR System?

Yes, you can use templates from other compatible systems, if you use the Thermo Fisher Cloud Design and Analysis module. Use the function “New Experiment --> From File” to import the .edt file.
I am having trouble connecting to my Thermo Fisher Cloud account, what could be wrong?

Check if the Cloud Monitor is on. Go to Settings, then Maintenance and Service, then Monitoring. Turn CloudSuite Monitor ‘ON'. This needs to be set to ‘ON', otherwise the instrument will not look out to connect to your account even if you are logged in.
Are my run files from the QuantStudio 3 or 5 Real-Time PCR System compatible with TaqMan Genotyper Software?

No. You can use the QuantStudio Design and Analysis Desktop Software, or for multi-plate analysis of genotyping data, please use the Applied Biosystems qPCR Analysis Module (GT application) with your Thermo Fisher Cloud account.
Are my run files from the QuantStudio 3 or 5 Real-Time PCR System compatible with ExpressionSuite Software?

Yes, ExpressionSuite Software v1.1 is compatible with files from the QuantStudio 3 and 5 Real-Time PCR Systems..
I have a qPCR master mix from another vendor that has ROX passive reference dye in a separate tube. How much should I add for my reactions in the QuantStudio 3 and 5 Real Time PCR Systems?

If you are using a master mix that has ROX provided in a separate tube, use a low ROX concentration (50 nM, final well volume).
How do I analyze multiple ddCt files together from a QuantStudio 3 or 5 Real-Time PCR System?

Use the Applied Biosystems qPCR Analysis Module (RQ application) with your Thermo Fisher Cloud account.
How do I start a qPCR run on the QuantStudio 3 or 5 Real-Time PCR System using the cloud?

1. Set up the run in the cloud from the Design and Analysis application within the Applied Biosystems qPCR Analysis Modules.
2. Save the experiment before starting a run: The file name was created from the experiment Name entered in the Properties tab. However, changes to the experiment Name in the Properties tab after the first save do not update the file name. To change the file name before a run, select Actions, then Save As.
3. Go to an available cloud-connected instrument, enter your PIN to access this file, and start the run by choosing ‘Open Template'. Following a run, view experiment details, run duration, and instrument and software information in the Run Control screen of the Run tab.
Can I start a run on the QuantStudio 3 or 5 Real-Time PCR System from the Thermo Fisher Cloud Design and Analysis application?

You can set up your run in the Design and Analysis application, but to actually start the run, you will have to go to the instrument touchscreen.
Can I monitor my qPCR run from the QuantStudio 3 or 5 Real-Time PCR System in real time?

Yes, you have several options for this. You can monitor the run in real time from:

- The instrument touchscreen
- The QuantStudio Design and Analysis Desktop Software
- Online using the Design and Analysis application within Thermo Fisher Connect
- Online using the Instrument Connect App on your Android or Apple mobile device - for more on this, please see here (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/quantstudio-3-5-real-time-pcr-system/connectivity-quantstudio-3-5-qpcr-data-analysis-software.html).

With the QuantStudio 3 or QuantStudio 5 Real-Time PCR System, I want my file to go to my Thermo Fisher Cloud account once the run is complete for analysis. Is there a way for it to automatically upload?

Yes. To have the run file automatically upload to your Thermo Fisher Cloud account once complete, you will need to set the file destination on the touchscreen, prior to starting the run:
1. Before you start the run, select the ‘Properties' tab from an open experiment
2. Touch ‘Edit', Data Destination - Choose USB or Cloud
Alternatively, you could use Cloud Connect Utility to have all files from a specific hard drive folder location automatically upload to the cloud (for use with the QuantStudio Design and Analysis Desktop Software).
How do I open the drawer in the QuantStudio 3 or QuantStudio 5 Real-Time PCR System?

The block drawer is opened and closed by using the ‘eject' button on the touchscreen. Do not push or pull on the instrument drawer.
When do I have to calibrate the QuantStudio 3 and QuantStudio 5 Real Time PCR Systems?

When your new QuantStudio 3 or QuantStudio 5 Real-Time PCR System arrives in your lab, it will be factory calibrated. The recommended calibration schedule is to recalibrate every 2 years.
Can I purchase or use the QuantStudio 3 or QuantStudio 5 Real Time PCR System without a computer?

Yes, you can purchase the QuantStudio 3 or QuantStudio 5 Real Time PCR System without a computer. In this configuration, you can run the instrument through the touchscreen or the Design and Analysis application from Thermo Fisher Cloud.
Do the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems have the ability for security and auditing software?

Yes, the QuantStudio 5 Real-Time PCR System has SAE (Security/Auditing/e-Signature) features that can be enabled in the QuantStudio Design and Analysis Desktop Software.
What is the difference between the Applied Biosystems QuantStudio 3 and Applied Biosystems QuantStudio 5 Real-Time PCR Systems?

The QuantStudio 3 Real-Time PCR System is a 4-color instrument with a 3-zone VeriFlex block, while the QuantStudio 5 Real-Time PCR System is a 6-color instrument with 6-VeriFlex zones in a 96-well block, (or 5-color format, no VeriFlex with 384-well block).

For Research Use Only. Not for use in diagnostic procedures.