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Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System, desktop

Catalog No. A52864
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Catalog No. A52864 Supplier Applied Biosystems™ Supplier No. A52864

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The Applied Biosystems QuantStudio Absolute Q Digital PCR System combines the power of digital PCR (dPCR) absolute quantification with the simplicity of a qPCR workflow.

The Applied Biosystems QuantStudio Absolute Q Digital PCR System combines the power of digital PCR (dPCR) absolute quantification with the simplicity of a qPCR workflow. With a single hands-on step that takes five minutes to complete, dPCR is now easier than ever. The QuantStudio Absolute Q system provides a high quality, consistent, and easy-to-use solution for researchers who want to join the quantification revolution.

Features of the QuantStudio Absolute Q system include:
• Easy and convenient qPCR-like workflow with only five minutes of hands-on time
• Reduced errors and manual inputs with all functions integrated into a single instrument
• Increased productivity with results in as little as 90 minutes
• Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
• QuantStudio Absolute Q Digital PCR software enables intuitive setup, monitoring, and analysis
- Security, auditing, and e-signature features for 21CFR part 11-compliance support
• Compatible with Absolute Q Liquid Biopsy Digital PCR assays for oncology applications, as well as our portfolio of TaqMan assays for gene expression, genetic variation, gene regulation, and other quantification experiments

Easy and convenient workflow
The QuantStudio Absolute Q system was designed for a simple and powerful dPCR experience. All the necessary steps for dPCR—compartmentalization, thermal cycling, and data acquisition—are integrated into a single instrument. The simple qPCR-like workflow can be completed with under 5 minutes of hands-on time.

Accomplish more, faster
The QuantStudio Absolute Q system can complete a run in less than 90 minutes.

Results you can trust
Consumable plates with patented microfluidic array technology allow for over 95% of the sample to be analyzed across over 20,000 consistently generated microchambers for optimal quantification of even rare targets.

Application versatility
With the ability to multiplex using up to four optical channels, the QuantStudio Absolute Q system enables more targets to be measured per sample, saving time and reagents. The Absolute Q dPCR platform is an ideal solution for any application requiring high sensitivity, precision, and accuracy.

Powerful and intuitive software
Absolute Q dPCR software is designed to complement our intuitive workflow to save time and effort. Parameters such as plate format, optical channels, and thermal conditions can be easily modified to start generating data quickly.

The clean and powerful data analysis interface provides a range of options for processing data. Whether visualizing multiplexed data or calculating concentrations across replicates, results can be exported for downstream analysis or compiled into an easy-to-read report in just a few clicks.

Included security, auditing and e-signature software features support compliance with 21CFR part 11 for regulated environments.

Peace of mind with Smart service
Minimize downtime with premium service offerings for the QuantStudio Absolute Q Digital PCR System. Smart Remote Support is an immersive collaboration tool that allows customers to quickly and efficiently show issues to Technical Support for resolution, helping reduce instrument downtime compared to on-site repair and helping minimize the need for on-site visits.

Specifications

Block Format Non-interchangeable
Content And Storage QuantStudio Absolute Q Digital PCR System, Desktop
For Use With (Equipment) QuantStudio Absolute Q MAP16 Plate
Product Line QuantStudio
For Use With (Application) Digital PCR
Shipping Condition Room Temperature
Quantity 1 system

Frequently Asked Questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.
What is the limit of detection for the QuantStudio Absolute Q Digital PCR System (Cat. No. A52864)?

The QuantStudio Absolute Q Digital PCR Instrument (Cat. No. A52864) uses QuantStudio Q MAP16 Digital PCR Plates that contain 16 digital PCR microreaction chamber arrays, each containing 20,480 fixed volume microreaction chambers of 432 ρL (0.000432 µL). If there is 1 copy of gene target in the tested sample, and the sample is using 1 array of the MAP16 plate, then the theoretical lower limit of detection (lowLOD) for the Absolute Q can be approximated by the following calculation:

lowLOD for Absolute Q = (1 copy)/ (0.000432 µL X 20,480 chambers) = 0.113 copies per µL

If a sample is run in triplicate, and there are 3 positive chambers, the lowLOD in practice for Absolute Q can be calculated using the following calculation:

lowLOD for Absolute Q = (3 copies)/ (0.000432 µL X 20,480 chambers) = 0.339 copies per µL

The QuantStudio Absolute Q has the sensitivity of detecting the presence of a single copy of target sequence within the 9 µL reaction volume, which would result in a detected concentration of ~0.1 copies per µL. This detection limit can be reduced even further by using the software’s digital pooling feature to increase the total analyzed volume. The ability of the instrument to detect at this level will be impacted by various components including the assay efficiency and reagents used in the reaction. The LOD is also dependent on a given assay’s specificity as well as the potential background fluorescence signal from the sample matrix, which may affect the LOD achieved in practice. The Absolute Q software’s unique false positive detection capability can enhance an assay’s performance, especially when low target concentrations are investigated.

It is important to note that the LOD should be determined empirically by the user for their specific experimental conditions.

What optical dyes does QuantStudio Absolute Q Digital PCR System (Cat. No. A52864) support?

QuantStudio Absolute Q Digital PCR System (Cat. No. A52864) supports the following dyes: FAM™ dye, VIC™ dye, HEX™ dye, ABY™ dye, ROX™ dye, CY™5 dye, and JUN™ dye.

Please refer to page 13 of the QuantStudio Absolute Q Digital PCR System User Guide for Installation, Use and Maintenance for more information on supported optical dyes and channel selection.

I finished running my experiment on the QuantStudio Absolute Q Digital PCR instrument but just realized that I had selected the wrong dyes during setup. Can I change the dyes that were used after the run?

If dye channels are turned off before the run, then data will not be collected for them as the run progresses. This means that changing the dyes after the run will not be possible.
I ran my experiment using the QuantStudio Absolute Q Digital PCR System but did not actually use dyes in all the channels. Is there a way to only see those dye channels that were used?

Unused dye channels can be turned off after the run by going to the SETUP tab and clicking EDIT SETUP and then EDIT GROUPS. Here, the Analysis can be changed to “Not Used.” After clicking SAVE twice, the data will be reanalyzed and the unused dye channels will not be present in the analysis.
I'm using the QuantStudio Absolute Q Digital PCR System and the software is saying that the concentration is NaN. What does that mean?

NaN stands for “not a number”. The software will display NaN instead of an actual number when it has detected some issue during the analysis of the array images.
With the QuantStudio Absolute Q Digital PCR instrument, the run data didn't automatically transfer after the run completed. How can I get the data?

We would recommend restarting the software. If the run still doesn't transfer, we would recommend closing the software, rebooting the instrument, and then starting the software. If further assistance is needed, please contact Technical Support at techsupport@thermofisher.com.
With the QuantStudio Absolute Q Digital PCR System, how much sample can be loaded into each well?

9 µL of sample is loaded into each well along with 15 µL of isolation buffer. Loading more or less of either the sample or isolation buffer will affect filling of the microchambers and the run results.
How do I know how much sample to use with the QuantStudio Absolute Q Digital PCR System?

Please see the sections on determining the optimal dilution in Chapter 2 of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025621_QS_Absolute_Q_Digital_PCR_System_UG_RUO_EN.pdf).

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How many samples can be run on the QuantStudio Absolute Q Digital PCR instrument at one time?

The QuantStudio Absolute Q MAP16 Digital PCR Plate can accommodate up to 16 individual samples. Loading the same sample in multiple microarrays as replicates or for the purpose of pooling results will decrease the number of individual samples that can be run on the same plate.
How many QuantStudio Absolute Q Digital PCR instruments can be managed at the same time using the QuantStudio Absolute Q Digital PCR Software?

QuantStudio Absolute Q Digital PCR Software can only manage one QuantStudio Absolute Q Digital PCR instrument at a time.
How long does a run on the QuantStudio Absolute Q Digital PCR System take?

A result can be provided in less than 90 mins depending on the protocol that is used.
Are there any special precautions when loading the QuantStudio Absolute Q MAP16 Digital PCR Plate?

Yes. Here are some precautions to take:
- Do not allow the pipette tip to come in contact with the bottom of the well or puncture the thin membrane that resides at the bottom of the well.
- Be careful not to induce bubbles when pipetting into the wells.
- Do not mix the isolation buffer and sample.
- Ensure that the gaskets are oriented the correct way and are pushed all the way on.

For more detailed instructions, please see the guidelines in Chapter 2 of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025621_QS_Absolute_Q_Digital_PCR_System_UG_RUO_EN.pdf).
What master mix can I use with the QuantStudio Absolute Q Digital PCR System?

The QuantStudio Absolute Q Digital PCR System is meant to be used with the Absolute Q DNA Digital PCR Master Mix (5X) (Cat. No. A52490) or Absolute Q 1-step RT-dPCR Master Mix (4X) (Cat. No. A55146). Other master mixes have not been validated with this system.
How do I find the .zst file for the QuantStudio Absolute Q Digital PCR System?

The .zst data files are not automatically generated. To create a .zst file, select the run by checking the box to the left of the run name and then click on the “Export Run” button that appears. You will be asked if that is the run you would like to export and then would need to select a location to export the file to.
What dyes are supported by the QuantStudio Absolute Q Digital PCR System?

The QuantStudio Absolute Q Digital PCR System has filters that support FAM, HEX (not field calibrated), VIC, ABY, ROX (QC channel), CY5, and JUN dyes.
Does the QuantStudio Absolute Q instrument use channels (e.g., ROX dye) to determine which compartments have a reaction?

Yes, ROX dye is the passive reference dye for QC (Quality Control).
Do I need to use the entire QuantStudio Absolute Q MAP16 Digital PCR Plate at one time?

No. Microarrays will be used in columns of 4 and unused columns can be used at a future time.
What does the QuantStudio Absolute Q MAP16 Digital PCR Plate look like?

The QuantStudio Absolute Q MAP16 Digital PCR Plate is a microinjection-molded plate which is 85.5 mm x 128 mm and consists of 16 microarrays. Each microarray consists of approximately 20,000 microchambers, each accommodating 432 pL (picoliter) volume of reaction. See image on page 29 of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025621_QS_Absolute_Q_Digital_PCR_System_UG_RUO_EN.pdf).
What are the electrical and environmental specifications for the QuantStudio Absolute Q Digital PCR System?

For QuantStudio Absolute Q instrument specifications, please see Appendix F of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025621_QS_Absolute_Q_Digital_PCR_System_UG_RUO_EN.pdf).
How many QuantStudio Absolute Q instruments can be managed through QuantStudio Absolute Q Software?

QuantStudio Absolute Q Software van only manage one QuantStudio Absolute Q instrument at a time.
Does the QuantStudio Absolute Q instrument use channels (e.g., ROX) to determine which compartments have a reaction?

Yes, ROX is the passive reference dye for QC (Quality Control).
For the QuantStudio Absolute Q Digital PCR System, what does MAP mean or stand for?

MAP stands for patented Microfluidic Array Plate technology. The QuantStudio Absolute Q Digital PCR instrument uses QuantStudio Absolute Q MAP16 Digital PCR Plates (MAP plates) for loading samples and running experiments. The MAP plates use microinjection-molded plate technology to overcome common reagent distribution challenges of inconsistency and high dead volume. This novel approach provides many benefits, including precise micro-chamber volume, consistent sample digitization, and greater than 95% of the loaded volume analyzed:
- 16 samples per plate
- 20,000 micro-chambers per reaction
- <5% dead volume
For the QuantStudio Absolute Q Digital PCR System, what is the volume per compartment?

Each microchamber holds 432 picoliters (pL).

For Research Use Only. Not for use in diagnostic procedures