DMEM-F-12 Cell Culture Media

DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.

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Gibco™ DMEM/F-12, HEPES, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability
Specifications

Cell Line	MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage	Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Phenol Red,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Glutamine,HEPES
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Cytiva HyClone™ DMEM/F12 1:1 Media

Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.

Pricing and Availability
Specifications

Content And Storage	2° to 8°C, away from light
Sterilization Method	0.1 μm Sterile-filtered
Product Type	DMEM/F12 1:1 Medium
Form	Liquid
Certifications/Compliance	ISO 9001:2000
Shelf Life	12 Months
Sterility	Sterile-filtered

Gibco™ DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage	Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Reduced Serum

Gibco™ DMEM/F-12, GlutaMAX™ supplement

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix

Used for culture of epithelial, endothelial, and granulosa cell types under low serum conditions. Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix has low levels of endotoxin, and absence of contamination.

Pricing and Availability
Specifications

Content And Storage	2° to 8°C
Product Type	DMEM and Ham's F-12, 50/50 Mix
Form	Liquid
Endotoxin Level	<0.25EU/mL
pH	7 to 7.4
Shelf Life	12 Months
Concentration	1 X
For Use With (Application)	To assess growth promotion and cytotoxicity characteristics
Sterility	Sterile

Gibco™ DMEM/F-12, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability

Gibco™ DMEM/F-12, powder, HEPES

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability

Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

Pricing and Availability

MP Biomedicals™ DMEM/Ham's F-12 Medium without L-glutamine, sodium bicarbonate

Powder; With 3151 mg/L dextrose

Pricing and Availability

Gibco™ DMEM/F-12, no glutamine

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability
Specifications

Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	12 Months

Gibco™ KnockOut™ DMEM/F-12

KnockOut™ DMEM/F-12

Pricing and Availability

Gibco™ DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Gibco™ CTS™ KnockOut™ DMEM/F-12

CTS KnockOut DMEM/F-12 CTS is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

Pricing and Availability

Gibco™ DMEM/F-12, powder

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Form	Powder
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

DMEM-F-12 Cell Culture Media

DMEM-F-12 Cell Culture Media

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Gibco™ DMEM/F-12, HEPES, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, GlutaMAX™ supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ KnockOut™ DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, HEPES Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ CTS™ KnockOut™ DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

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Gibco™ DMEM/F-12, HEPES, no phenol red

Gibco™ DMEM/F-12

Gibco™ Advanced DMEM/F-12

Gibco™ DMEM/F-12, GlutaMAX™ supplement

Gibco™ DMEM/F-12, no phenol red

Gibco™ KnockOut™ DMEM/F-12

Gibco™ DMEM/F-12, HEPES

Gibco™ CTS™ KnockOut™ DMEM/F-12