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Gibco™ DMEM/F-12, no glutamine

Catalog No. 361045737
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500 mL
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361045737 500 mL
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Catalog No. 361045737 Supplier Gibco™ Supplier No. 21331020
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Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With: Phenol Red

Without: L-glutamine, HEPES

Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Specifications

Cell Type Mammalian Cells
Concentration 1 X
Form Liquid
Product Type Dulbecco's Modified Eagle Medium (DMEM)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives High Glucose, Phenol Red, Sodium Pyruvate
Without Additives No Glutamine, No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Type Mammalian Cell Culture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.