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Gibco™ DMEM/F-12, no glutamine
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Supplier: Gibco™ 21331020
Description
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: Phenol Red
Without: L-glutamine, HEPES
Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.
DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Chemical Identifiers
| Liquid | |
| No Glutamine, No HEPES | |
| cGMP-compliant under the ISO 13485 standard |
Specifications
| Mammalian Cells | |
| Liquid | |
| Standard Serum Supplementation | |
| High Glucose, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| 1 X | |
| Dulbecco's Modified Eagle Medium (DMEM) | |
| Sterile-filtered | |
| No Glutamine, No HEPES | |
| Gibco | |
| Room Temperature | |
| Mammalian Cell Culture |
Safety and Handling
ShelfLife : 12 Months
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.