DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Supplier: Gibco™ 11320033
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: L-glutamine, Phenol Red
The complete formulation is available.
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
|Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
|Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
|DMEM (Dulbecco's Modified Eagle Medium)/F-12
|cGMP-compliant under the ISO 13485 standard
|MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
|Standard Serum Supplementation
|High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.