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Additional Cell Culture Reagents and Supplements

A broad array of reagents that support specific cell culture operations, processes, and analytics. Products included may promote adherence and spreading, nurture specific cell lines, or support transfection processes.
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1
Promotions Available

Cytiva HyClone™ Dulbecco's Phosphate Buffered Saline, Solution

Buffer cell culture with PBS in solution.

EDGE
Regulatory Status RUO
Content And Storage 15° to 30°C
Sterilization Method 0.1 μm Sterile-filtered
Product Type Dulbecco's Phosphate Buffered Saline
Form Liquid
Endotoxin Level ≤0.025EU/mL
pH 7 to 7.6
Shelf Life 24 Months
Certifications/Compliance Manufactured under cGMP and ISO 9001 certified facilities
For Use With (Application) Serves as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time
Osmolality 276±10mOsm/kg H2O
Sterility Sterile
2
Promotions Available

Cytiva HyClone™ Phosphate Buffered Saline (PBS)

Buffer cell culture growth with PBS

EDGE
Content And Storage 15° to 30°C
Sterilization Method 0.1 μm Sterile-filtered
Product Type Phosphate Buffered Saline (PBS)
Form Liquid
Without Additives No Calcium,No Magnesium,No Phenol Red
Endotoxin Level =1.0EU/mL
pH 7.0 to 7.2
Certifications/Compliance cGMP and ISO 9001:2000 Guidelines
3
Promotions Available

Cytiva HyClone™ 100mM Sodium Pyruvate Solution

Filter sterilized. HyClone™ 100mM Sodium Pyruvate solution is an easily accessible carbohydrate source that may also have protective effects against hydrogen peroxide.

EDGE
4
Promotions Available

Gibco™ B-27™ Supplement (50X), serum free  DFS Item
GREENER_CHOICE

The original Gibco B-27 Supplement, optimized for serum-free culture of neurons, supports the growth and viability of neurons and many different cell types.

Content And Storage Store in freezer (-5 to -20°C) and protect from light.
Product Type Cell Culture Supplement
Form Liquid
Tested For Endotoxin, Performance, Sterility, Mycoplasma
Classification Serum-free
Culture Type Mammalian Cell Culture
Shelf Life 12 Months
Sterility Sterile
Serum Level Serum-Free
Cell Type Neural Cells
5

Corning™ Nu-Serum™ IV Growth Serum Replacements
GREENER_CHOICE

Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture

6

Corning™ Nu-Serum™ Growth Medium Supplement
GREENER_CHOICE

Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture

Content And Storage -20°C
Product Type Growth Supplement
7
Promotions Available

Cytiva HyClone™ HEPES Solution

Maintains physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration). HyClone™ HEPES Solution is part of high-performance line of nutritional and growth supplements.

EDGE
8

MilliporeSigma™ Poly-L-Lysine Solution

Used as coating to enhance cell attachment and adhesion to both plasticware and glass surfaces

EDGE
9

MilliporeSigma™ Polybrene Infection/Transfection Reagent

A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.

EDGE
10

Gibco™ GlutaMAX™ Supplement  DFS Item
GREENER_CHOICE

GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine. It exhibits increased stability over L-glutamine leading to improved cellular health.

Content And Storage Storage conditions: 15°C to 30°C
Alternate storage conditions: -5°C to -20°C and 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type GlutaMax
Form Liquid
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Culture Type Mammalian Cell Culture
Shelf Life 24 Months
For Use With (Application) Clinical Research, Translational Research
Sterility Sterile-filtered
Product Line Gibco, GlutaMAX
Cell Type Mammalian Cells
11

Gibco™ Poly-D-Lysine  DFS Item

Poly-D-Lysine is an extracellular matrix used to facilitate neural cell adhesion to tissue culture-treated plastic and glass surfaces. When used on Nunc plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

13

Gibco™ MEM Non-Essential Amino Acids Solution (100X)  DFS Item
GREENER_CHOICE

Gibco™ MEM Non-Essential Amino Acids are used as a supplement for cell culture medium, to increase cell growth and viability.

Content And Storage Store at 2–8°C. Protect from light.
Product Type Amino Acid Supplement
Form Liquid
pH 0.9
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 18 Months
Concentration 100X
Sterility Sterile-filtered
Cell Type Mammalian Cells
14

Gibco™ Trypan Blue Solution, 0.4%  DFS Item
GREENER_CHOICE

Routinely used as a cell stain to assess cell viability using the dye exclusion test

15

Gibco™ 2-Mercaptoethanol  DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals.