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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1 – 15 de 93 résultats
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Thermo Scientific™ DH5α Competent Cells

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

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Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

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Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.

4

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Résistance aux antibiotiques des bactéries Yes (Streptomycin)
Clonage d’ADN méthylé Yes (mcrA)
Type de produit Chemically Competent Cells
Plasmide High Copy Plasmid
Résistant au phage T1 (tonA) No
Espèces E. coli (K12)
Conditions d’expédition Dry Ice
Compatibilité à haut débit Low
Format Tube
Sélection bleue / blanche Yes (lacZΔM15)
Niveau d’efficacité de la transformation High Efficiency (>1 x 109 cfu/μg)
Gamme de produits One Shot
Contient l’épisome F No
Réduit la recombinaison Yes (recA1)
Améliore la qualité des plasmides Yes (endA1)
Préparation de l’ADN non méthylé No
5

MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
6

MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLacI Competent Cells

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Efficacité Guaranteed efficiency: 2 x 106 cfu/μg
Contenu et stockage ≤-70°C
7

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Singles Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
8

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
9
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Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.

10

Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

11

Invitrogen™ Tali™ Viability Kit - Dead Cell Red

Validated for use with the Tali Image-Based Cytometer

12

Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells

One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.

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Invitrogen™ Tali™ Cell Cycle Kit

Convenient and accurate determination of the percentage of cells in each phase of the cell cycle

14

Gibco™ Expi293™ Met (–) Expression Medium

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.

15

Thermo Scientific™ BL21(DE3) Competent Cells

Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.