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MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLysS Singles™ Competent Cells

Catalog No. 714014
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22 Reactions
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714014 22 Reactions
714013 11 Reactions
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Catalog No. 714014 Supplier MilliporeSigma™ Supplier No. 714014
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Description

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Specifications

Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
Content And Storage ≤-70°C
Quantity 22 Reactions