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cDNA and ORF Libraries

Collections of defined cDNA fragments and open reading frame DNA sequences that have been cloned into vectors for the purpose of easy identification and isolation for further study. Ideal for use in cloning experiments.
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1

Invitrogen™ 3' RACE System for Rapid Amplification of cDNA Ends

Converts up to 1μg of total RNA preparation or 100ng of control RNA into cDNA in one reaction

2

Invitrogen™ 5' RACE System for Rapid Amplification of cDNA Ends, version 2.0

Suitable for rapid amplification of cDNA ends (RACE) between defined point in mRNA and the 5' end

3

Invitrogen™ cDNA Size Fractionation Columns

Offers efficient and convenient method for size-fractioning double-stranded cDNA (1)

4

Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Température de réaction optimale 42°C
Format Kit
Type de produit RLM-RACE Kit
Gamme de produits Ambion™, FirstChoice™
Type de produit final cDNA
Transcriptase inverse M-MLV
À utiliser avec (application) cDNA Libraries & Library Construction
Contenu et stockage

•FastAP™ Thermosensitive Alkaline Phosphatase
•10X FastAP Buffer, Tobacco Acid Pyrophosphatase
•10X TAP Buffer, T4 RNA Ligase
•10X T4 RNA Ligase Buffer
•M-MLV Reverse Transcriptase
•RNase Inhibitor
•10X RT Buffer
•2.5 mM dNTP Mix
•Mouse Thymus RNA (1 mg/ml)
•Random Decamers
•5' RACE Adapter
•3' RACE Adapter
•5' RACE Outer Primer
•5' RACE Inner Primer
•3' RACE Outer Primer
•3' RACE Inner Primer
•5' RACE Outer Control Primer
•5' RACE Inner Control Primer
•5' PCR Control Primer
•3' RACE Control Primer
•Ammonium Acetate Stop Solution
•Components should be stored at -20°C. Nuclease-free Water may be stored at any temperature.
5

Thermo Scientific™ MuSeek Library Preparation Kit, Illumina compatible

Illumina-compatible next-gen sequencing (NGS) libraries from as little as 50ng of starting DNA. DNA fragmentation and transposon-tagging are accomplished in one step.

6

Promega pF4A CMV Flexi™ Vector

Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker

7

Promega pF9A CMV hRluc-neo Flexi™ Vector

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

8

Promega pF1K T7 Flexi™ Vector

These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.

9

Promega pFN2K (GST) Flexi™ Vector

These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

10

Promega pF4K CMV Flexi™ Vector

Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker

11

Promega pF5K CMV-neo Flexi™ Vector

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

12

Promega HaloTag™ Cloning Starter System

HaloTag™ Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag™ expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag™ Flexi CMV Deletion Vectors.

13

Promega pF3K WG (BYDV) Flexi™ Vector

Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.

14

Promega pFC14K HaloTag™ CMV Flexi™ Vector

Vectors designed for expression of C-terminal-tagged HaloTag™ fusion proteins in mammalian cells. Once expressed, use the HaloTag™ fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag™ Ligands.