Modifying Enzymes

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Invitrogen™ DNA-free™ DNA Removal Kit  

Safely eliminate DNA contamination from RNA samples

Invitrogen™ TURBO DNA-free™ Kit  

Ideal tool for clearing residual DNA

MilliporeSigma™ Upstate p300, HAT Domain Recombinant Enzyme

Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300.

MilliporeSigma™ Novagen™ Chicken Egg White Lysozyme Solution

For purification of both DNA, protein, and Gram-positive bacterial lysis

Applied Biosystems™ Exonuclease I, high concentration, (20 units/μL)  

Exonuclease I can be used to measure the endonucleolytic cleavage of covalently closed circular single-stranded DNA reacted with an endonuclease of interest.

Applied Biosystems™ Exonuclease I, standard concentration, (10 units/μL)  

Exonuclease I is particularly useful in preparing the products of PCR for applications involving sequencing or labeling methods.

Thermo Scientific Chemicals Pectinase, Aspergillus niger

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

MilliporeSigma™ Novagen™ KOD XL DNA Polymerase

For PCR amplification of long strand and GC-rich DNA templates

Invitrogen™ Anza™ Alkaline Phosphatase  

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Invitrogen™ Anza™ T4 DNA Ligase Master Mix  

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

MilliporeSigma™ Novagen™ KOD Xtreme™ Hot Start DNA Polymerase

For challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates

Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)  

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

MilliporeSigma™ Novagen™ T4 DNA Polymerase, LIC-Qualified

For any ligation or cloning application

Thermo Scientific Chemicals Peroxidase, horseradish

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.