Modifying Enzymes
Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)
MilliporeSigma™ Calbiochem™ RNase A, Bovine Pancreas
For selective degradation of RNA
Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
Invitrogen™ UltraPure™ Glycogen
Used as carrier for nucleic acid precipitation in ethanol
MilliporeSigma™ Calbiochem™ Human Recombinant Topoisomerase I, His.Tag™, Wild Type
Recombinant, human topoisomerase I fused to a His Tag
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Invitrogen™ Bovine Serum Albumin
Suitable for use as enzyme stabilizer
Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix
Catalyzes in vitro recombination of PCR products to generate entry clones
Invitrogen™ M13KO7 Helper Phage
Suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication
Thermo Scientific™ RNase T1 (1000 U/μL)
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Invitrogen™ Proteinase K Powder
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL)
Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.
Invitrogen™ AcTEV™ Protease
Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins