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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
Séquençage de l’ADN (221)
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Kits de clonage par PCR (104)
Clonage d’ADN, cartographie et mutagenèse (83)
ADN plasmide (43)

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2

Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ INVαF' Chemically Competent E. coli  DFS Item

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Souche bactérienne ou de levures INVαF ́
Format Kit
Type de produit Cloning Kit
Vecteur pCRII
Gamme de produits One Shot
Comprend Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Type de cellule Chemically Competent E. coli
Accélérateur T7, SP6
Méthode de clonage TA Cloning
À utiliser avec (application) PCR Cloning
Contenu et stockage This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
4

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Type de cellule Chemically Competent
Accélérateur SP6
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
5

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Souche bactérienne ou de levures DH5α
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, T3
Méthode de clonage TOPO™-TA
À utiliser avec (application) Chromatin Biology
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
6

Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit  DFS Item

Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products

7

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Résistance aux antibiotiques des bactéries Ampicillin (AmpR)
Format Kit
Vecteur pJET1.2/blunt
Gamme de produits CloneJET
Type d’échantillon PCR Products
Méthode de clonage Restriction Enzyme⁄ MCS, Blunt PCR
À utiliser avec (application) PCR Cloning
8

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

9

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, T3
Méthode de clonage TOPO™-TA
À utiliser avec (application) Chromatin Biology
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
10

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

11

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells  DFS Item

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Souche bactérienne ou de levures Not Included
Format Kit
Type de produit Cloning Kit
Vecteur pCR2.1
Gamme de produits TA Cloning
Accélérateur T7
Méthode de clonage TA Cloning
À utiliser avec (application) PCR Cloning
Contenu et stockage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
12

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

13

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli  DFS Item

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

14

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit  DFS Item

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

15

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing