Gel Electrophoresis Buffers and Diluents

Thermo Scientific™ Citrate-buffered saline (20X)

Invitrogen™ SSPE (20X), RNase-free  

Molecular biology grade, Invitrogen 20X SSPE solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.

Acetate Buffer pH 4.5 DILUT-IT™ Dissolution Media Concentrate, J.T. Baker™

Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (6X)

Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.

Invitrogen™ ZOOM™ Carrier Ampholytes pH 9-11  

Small, soluble molecules with both positive and negative charge groups

Invitrogen™ ZOOM™ Carrier Ampholytes pH 4-7  

Small, soluble molecules with both positive and negative charge groups

Invitrogen™ NuPAGE™ Antioxidant  

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (4X)

Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.

Thermo Scientific Chemicals Acetate Buffer (1 M, pH 3.5)

CAS: 127-09-3

20X Saline-Sodium-Phosphate-EDTA (SSPE) Liquid Buffer, Corning™

Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)  

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.

LiChropur™ Sodium Phosphate Monobasic, MilliporeSigma™ Supelco™

CAS: 7558-80-7

Thermo Scientific™ Pierce™ Endotoxin-Free Magnesium Chloride (MgCl₂) (10 mM)

Pierce Endotoxin-Free MgCl₂ buffer is used to prepare samples for any LAL assay. As a diluent, it will eliminate the inhibitory chelation effects of EDTA. Contains <0.005 EU/mL endotoxin.

Invitrogen™ NativePAGE™ Running Buffer Kit  

Contains the NativePAGE Running Buffer (20X)

Invitrogen™ NativePAGE™ Sample Prep Kit  

Includes sample preparation reagents for native gel electrophoresis