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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
2

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

3

Invitrogen™ Novex™ Tris-Glycine SDS Buffer Kit  DFS Item

Made with high-purity reagents and are strictly quality controlled

4

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

5

Invitrogen™ Bolt™ Antioxidant  DFS Item

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

7

Invitrogen™ Tris-Glycine Transfer Buffer (25X)  DFS Item

Made with high-purity reagents and are strictly quality controlled

8

MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit

Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.

9

MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma

Ready to use blocking reagent for western blotting immunoassays.

10

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007  DFS Item

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

11

Invitrogen™ iBind™ Western Starter Kit  DFS Item

Complete kit for automated Western processing

13

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric
14

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

15

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)  DFS Item

Optimized, easy-to-use blocker and primary antibody diluent system