General Western Blot Reagents
General Western Blot Reagents
MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma
Ready to use blocking reagent for western blotting immunoassays.
MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit
Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.
MilliporeSigma™ Calbiochem™ MOWIOL™ 4-88 Reagent
Used for mounting cells on slides for detection of cell-surface antigens.
MilliporeSigma™ Calbiochem™ ImmunoPen™
Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
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Invitrogen™ XCell II™ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
Invitrogen™ Novex™ Reversible Membrane Protein Stain Kit
Used for determining protein transfer efficiency after blotting
Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer
Invitrogen™ Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675
Use with Novex Tricine Gels
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Thermo Scientific™ Pierce™ Western Blot Signal Enhancer
Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.
Thermo Scientific™ Pierce™ 20X TBS Tween™ 20 Buffer
Prepare TBS-Tween (TBS-T) wash buffers or blocking buffers for ELISA, Western blotting and other immunoassays with this space-saving stock solution.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer
Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
Invitrogen™ Novex™ Tris-Glycine SDS Buffer Kit
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
All buffers are made with high-purity reagents and are strictly quality controlled
Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels
Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent
Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Invitrogen™ Incubation Tray, 10 x 14 cm
Enables convenient and even staining of E-PAGE™ blots
Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ Novex™ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371
Optimized for using with Novex pH 3-7 IEF gels
Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer
Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF
Invitrogen™ ZOOM™ Cathode Buffer 10X pH 9-12
Use with ZOOM IPG Strips
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