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Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)

Catalog No. WB7050
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WB7050 80 mL
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Catalog No. WB7050 Supplier Invitrogen™ Supplier No. WB7050
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Description

Includes

80 mL of Blocker/Diluent (Part A); 80 mL of Blocker/Diluent (Part B)

Optimized, easy-to-use blocker and primary antibody diluent system

Yields low background/high signal western blot detection on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. Sufficient reagents for 20 mini-blots.

Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

Product Line WesternBreeze
Membrane Compatibility Nitrocellulose, PVDF
Product Type Blocker and Diluent
Buffer Blotting Buffers
Content And Storage 80 ml of Blocker/Diluent (Part A);
80 ml of Blocker/Diluent (Part B).

Store at +4°C.
Quantity 80 mL

Frequently Asked Questions (FAQs)

Why is the actual band size on a western blot different from the predicted size of the protein?

Western blotting is based on the separation of proteins by their size on a gel. However, migration of proteins through the gel matrix is also affected by other factors, which may cause the observed band size to be different from the predicted size.

Common causes are:
-Post-translational modification; for example phosphorylation and glycosylation increase the size of the protein
-Post-translation cleavage; many proteins are synthesized as precursor proteins, and then cleaved to give the active form
-Multimers, for example dimerization of a protein. This is usually prevented under reducing conditions, although strong interactions can result in the appearance of higher bands
-Splice variants; alternative splicing may result in different sized proteins being produced from the same gene
-Relative charge; the composition of amino acids (charged vs. non-charged)
What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).
Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chemiluminescent Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)
- Cat. No. WB7003 (Antibody Wash)

Note: Cat. Nos. WB7001 and WB7002 may be ordered by clicking the Quick Order button located at the top right of any page on our website.
Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chromogenic Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7003 (Antibody Wash)
- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)

For Research Use Only. Not for use in diagnostic procedures.