Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Optimze cDNA synthesis with these random hexamer primers, oligo(dT)₁₈ primers and anchored oligo dT primers.

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C

Primers for PCR amplification that complement many vectors

Truly random primers suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase

Use for first-strand cDNA synthesis with a reverse transcriptase.

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Provided at a stock concentration of 50μM

Used for priming and reverse transcription of polyadenylated (poly A+) mRNA

Used for first-strand synthesis of cDNAs, using poly-adenylated mRNA as template

Ambion pUC 19 DNA is digested to completion with Sau3A I

Thermo Scientific™ Oligo(dT)₁₈ Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

Primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC)

Contain mixtures of linearized plasmids ready for use as templates as part of in vitro transcription reactions for synthesis of labeled RNA molecular size standards

Ambion Lambda DNA is digested to completion with Hind III

Ideal for use as substrates as part of in vitro transcription reactions

Ideal for use as substrates as part of in vitro transcription reactions

Contain mixtures of linearized plasmids ready for use as templates during in vitro transcription reactions for synthesis of labeled RNA molecular size standards

Provided at a stock concentration of 50μM, and functionally tested using the RETROscript™ Kit

Ideal for use as substrates as part of in vitro transcription reactions

When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups

Ambion Modified nucleotides confer unique characteristics to the RNA molecules into which they are incorporated

Template primers

Perform highly efficient random priming of DNA synthesis reactions with this mixture of single-stranded random oligonucleotides.

Accurately sequence DNA with M13 pUC sequencing primers, single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends.

These oligonucleotides contain consensus DNA-binding sites for individual transcription factors and have 5′ OH blunt ends, allowing labeling to high specific activity with T4 PNK.

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.