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Ion Torrent™ IonCode™ Barcode Adapters 1-96 Kit

Catalog No. A29747
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A29747 960 Reactions
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Catalog No. A29747 Supplier Ion Torrent™ Supplier No. A29747
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Description

The IonCode Barcode Adapters 1-96 Kit provides a set of 96 unique barcode adapters specifically designed for optimal performance with the Ion GeneStudio S5, Ion PGM, and Ion Proton semiconductor sequencers.

The IonCode Barcode Adapters 1-96 Kit provides a set of 96 unique barcode adapters specifically designed for optimal performance with the Ion GeneStudio S5, Ion PGM, and Ion Proton semiconductor sequencers. When used in combination with the Ion AmpliSeq Library Kit PLUS or Ion AmpliSeq Library Kit 2.0, this kit enables users to pool up to 96 amplicon libraries and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment.

Features of the IonCode Barcode Adapters 1-96 Kit include:

  • Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes
  • Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
  • Robust error correction built in by design for added confidence in sample identification

Multiplexing with barcode adapters enables higher throughput

Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias.

Sequence- and flow-optimized for increased performance and efficiency

Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design

Built-in error correction prevents the possibility of incorrect sample assignment.

Specifications

Format Kit
For Use With (Equipment) Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
Product Type Barcode Adapter Kit
Content And Storage 1 x 96-well plate, 20 μL per well

Store in freezer (-5°C to -30°C).
Shipping Condition Dry Ice
Quantity 960 Reactions
Nucleic Acid Type DNA, RNA
For Use With (Application) For Use with Target Ampliseq DNA and RNA Library Preparation

Frequently Asked Questions (FAQs)

What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.

How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.
In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.
What are the shipping and storage conditions for IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.
What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Library bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Library bundle (Cat. No. A46495) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, and the IonCode Barcode Adapters for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 48 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.
Can I use the AmpliSeq_Microbiome_Health_ Analysis plugin for Torrent Suite Software and Ion Reporter Software workflows for my custom 16S or target species AmpliSeq panel?

The Torrent Suite AmpliSeq_Microbiome_Health_ Analysis plugin and Ion Reporter Software workflows were developed and validated exclusively for the Ion AmpliSeq Microbiome Health Research Kit. Open source analysis tools may be available for your custom solutions.
Can I customize the list of target species for my microbiome diversity research project?

Customization solutions are available through AmpliSeq custom designs (Ampliseq.com), where you can customize your own assays for any species of your interest. For details, please contact your local Field Applications Scientist (FAS).
Can the Ion AmpliSeq Microbiome Health Research Kit be used to investigate the human response to SARS-CoV-2 infection?

Several scientific publications have demonstrated the key role played by the gut microbial community in infection susceptibility and severity, including for SARS-CoV-2. The Ion AmpliSeq Microbiome Health Research Kit targets 73 species known to be associated with immune-related diseases, including a large number of known bacterial species with antiviral or immune-modulating properties.
What is the Ion AmpliSeq Microbiome Health Research Kit?

The Ion AmpliSeq Microbiome Health Research Kit is a targeted next-generation sequencing (NGS) assay that enables the profiling of the human gut microbiome by targeting eight of nine 16S hypervariable regions of 16S rRNA gene (16S rRNA Gene Pool) and allows for the accurate detection of 73 key bacterial species associated with human disease (Target Species Pool). The assay was validated on the Ion Chef and Ion GeneStudio systems and is available in different configurations, with only primers, primers and library prep reagents, or library prep, templating, and sequencing bundles. Check with your local Sales Rep for the best configuration for your experimental needs.
What is a microbiome?

A microbiome is a community of microorganisms living together in a particular habitat, for example the gut.
With the Ion AmpliSeq Microbiome Health Research Kit, I am seeing a high percentage (>15%) of low quality ISPs. Why is this?

- The overall library concentration may have been too low. We recommend renormalizing the individual libraries to 50 pM concentration and combining into a new library pool.
With the Ion AmpliSeq Microbiome Health Research Kit, I am seeing a high percentage (>40%) of poyclonal ISPs. Why is this?

- The overall library concentration may have been too high. We recommend diluting the library pool to 50 pM concentration.
- The individual library concentrations may have been too high. We recommend diluting the individual libraries to 50 pM concentration.

With the Ion AmpliSeq Microbiome Health Research Kit, I am seeing that the number of total reads per library is imbalanced. Why is this?

- The individual libraries may have been normalized to different concentrations. We recommend renormalizing the individual libraries to 50 pM concentration and combining into a new library pool.
- The individual libraries may have been misquantified. We recommend requantifying the individual libraries, then normalizing the individual libraries to 50 pM concentration and combining into a new library pool.

With the Ion AmpliSeq Microbiome Health Research Kit, I am seeing a low number of total reads for the chip run. Why is this?

The overall library input into templating may have been low. We recommend renormalizing the individual libraries to 50 pM concentration and combining into a new library pool.
Why is my library concentration high with the Ion AmpliSeq Microbiome Health Research Kit?

The sample DNA may have been misquantified. We recommend requantifying the sample DNA using the Qubit dsDNA HS Assay Kit and Qubit Fluorometer.
Note: Library yield is not indicative of quality.
Why is my library concentration low with the Ion AmpliSeq Microbiome Health Research Kit?

Here are possible causes and solutions:
-The sample DNA may have been misquantified. We recommend requantifying the sample DNA using the Qubit dsDNA HS Assay Kit and Qubit Fluorometer.
-The sample quality may have been low. We recommend reextracting the sample DNA using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit or adding more sample DNA to the amplification reaction.
-The PCR, digestion, or ligation reactions may have been inefficient. We recommend ensuring proper dispensing and mixing of viscous components at each step.
-The AMPure XP beads may have been overdried. We recommend not drying the AMPure XP beads for more than 5 mins.
Note: Library yield is not indicative of quality.
How many samples from the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle can I run on the Ion 550 Chip?

Up to 96 paired libraries (48 samples) can be combined and loaded onto a single Ion 550 Chip.
What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 540 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 540 bundle (Cat. No. A46496) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, IonCode Barcode Adapters, the Ion 540 Kit - Chef, and the Ion 540 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 256 research samples.

How much of each sample is needed for the Ion AmpliSeq Microbiome Health Research Kit?

The minimum sample input is 1 ng/pool for the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool and 10 ng/pool for the Ion AmpliSeq Microbiome Health Research - Target Species Pool.
What types of sample is the Ion AmpliSeq Microbiome Health Research Kit compatible with?

The Ion AmpliSeq Microbiome Health Research Kit was validated using DNA isolated from human stool samples to generate libraries for templating and sequencing on the Ion Chef and Ion GeneStudio systems. Different sample types and platform configurations may work but need to be validated by each lab. Contact your local Field Applications Scientist for assistance.
What is the Ion AmpliSeq Microbiome Health Research Kit, Ion 540 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 540 bundle (Cat. No. A46496) is a targeted next-generation sequencing (NGS) assay that enables the profiling of the human gut microbiome by targeting eight of nine 16S hypervariable regions of 16S rRNA gene (16S rRNA Gene Pool ) and allows for the accurate detection of 73 key bacterial species associated with human disease (Target Species Pool). This bundle allows templating and sequencing with the Ion 540 Chip to be done on the Ion Chef and Ion GeneStudio systems.

For Research Use Only. Not for use in diagnostic procedures