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Applied Biosystems™ Oligo d(T)16 (50 μM)
Description
Includes
Oligo(dT)16 primer is supplied in 100 μL of 10 mM TE, pH 7.0, at a concentration of 50 μM.
Invitrogen Oligo(dT)16 Primer is a homo-oligomeric deoxyribonucleotide poly(dT) that is non-phosphorylated and chemically synthesized. It is used for priming and reverse transcription of polyadenylated poly(A)+ mRNA.
Five nmoles of Oligo (dT)16 Primer is supplied in 100 μL of 10 mM TE, pH 7.0, at a concentration of 50 μM.
Order Info
Shipping Conditions: Dry Ice
Specifications
Specifications
| Product Type | Primer |
| Content And Storage | • Oligo(dT)16 Primer (100 μL at 50 μM) Store at –10 to –30°C. Aliquot to avoid repeated freezing and thawing. |
| Purification Method | Reverse Phase HPLC |
| Shipping Condition | Dry Ice |
| Concentration | 50 μM |
| Primer | Oligo dT |
| Quantity | 5 nmol, 100 μL |
| For Use With (Application) | Reverse Transcription |
| Form | Liquid |
Frequently Asked Questions (FAQs)
No. The reverse transcription of total RNA to cDNA must be done with random hexamers or random primers (or a mixture of random and oligo dT primers) because the 18S rRNA assay cannot evaluate poly A+ purified RNA samples as most of the ribosomal RNA has been removed. The plate has been designed to evaluate only total RNA.
For Research Use Only. Not for use in diagnostic procedures.