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Invitrogen™ Random Primers DFS Item

Truly random primers suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates

Manufacturer:  Invitrogen™ LS48190011

Catalog No. 48190011



Description

Description

Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer.

  • Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a Random Primers labeling reaction
  • Supplied at 3μg/μL in 3mM Tris-HCl (pH 7.0), 0.2mM EDTA

PCR and Real-Time PCR, RT-PCR, Reverse Transcription, Two-Step RT-PCR

Order Info

Shipping Conditions: Approved for shipment on Wet or Dry Ice

Specifications

Specifications

100 µL of Random Primers are supplied at a concentration of 3 µg/µL in 3 mM Tris-HCl (pH 7.0), 0.2 mM EDTA. Store at -20°C. Guaranteed stable for 6 months when properly stored.
9 A260 units
Random Primers
Liquid
Approved for shipment on Wet or Dry Ice
Documents

For Research Use Only. Not for use in diagnostic procedures.