Standard PCR Reagents and Kits

Standard PCR Reagents and Kits
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Invitrogenâ„¢ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
PCR Method | qPCR,Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | Taq DNA Polymerase |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Thermo Scientificâ„¢ DreamTaq PCR Master Mixes (2X)
Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.
Shipping Condition | Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
For Use With (Application) | Standard PCR |
Thermo Scientificâ„¢ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Standard PCR |
Invitrogenâ„¢ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Shipping Condition | Wet or Dry Ice |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | Taq DNA Polymerase |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Invitrogenâ„¢ PCR SuperMix
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Taq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | Separate Components |
Product Type | Taq DNA Polymerase (Recombinant) |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
Applied Biosystemsâ„¢ AmpliTaqâ„¢ DNA Polymerase with Buffer I or Buffer II
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Shipping Condition | Dry Ice |
---|---|
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq DNA Polymerase |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Applied Biosystemsâ„¢ AmpEraseâ„¢ Uracil N-Glycosylase (UNG)
Enzyme removes any uracil incorporated into single- or double-stranded DNA
Applied Biosystemsâ„¢ GeneAmpâ„¢ 10X PCR Buffer II & MgCl2
Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Polymerase | AmpliTaq DNA Polymerase |
---|---|
Fidelity (vs. Taq) | 1X |
Reaction Format | Kit |
Product Type | PCR Buffer |
Concentration | 10X |
For Use With (Application) | Standard PCR |
Applied Biosystemsâ„¢ AmpliTaqâ„¢ DNA Polymerase, LD (Low DNA)
Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Purity or Quality Grade | LD (Low DNA) |
Quantity | 250 units |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq DNA Polymerase |
No. of Reactions | 200 Reactions |
Format | Stand-alone enzyme |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | No |
Starting Material | DNA |
Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
Polymerase | Taq DNA Polymerase |
---|---|
Product Type | DNA Polymerase |
Concentration | 5 U/μL |
For Use With (Application) | Standard PCR |
Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
Applied Biosystemsâ„¢ GeneAmpâ„¢ Fast PCR Master Mix (2X), with or without protocol
The GeneAmp Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti 96-Well Thermal Cycler.
GC-Rich PCR Performance | Medium |
---|---|
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 2X |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq DNA Polymerase |
No. of Reactions | 250 Reactions |
Format | Tube |
Volume | 0.1 mL or 0.2 mL |
Size (Final Product) | 2 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | Fast PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Applied Biosystemsâ„¢ MicroSEQâ„¢ Full Gene 16S rDNA PCR Kit
The MicroSEQ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria.