Protein Labeling Reagents
Protein Labeling Reagents
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientific™ Pierce™ Dye Removal Columns
Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.
Molecular Probes™ Alexa Fluor™ 488 Azide (Alexa Fluor™ 488 5-Carboxamido-(6-Azidohexanyl), Bis(Triethylammonium Salt)), 5-isomer
The green-fluorescent Alexa Fluor™ 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction.
Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay.
Molecular Probes™ Dansyl Ethylenediamine, 5-Dimethylaminonaphthalene-1-(N-(2-Aminoethyl))sulfonamide
5-dimethylaminonaphthalene-1-(N-(2-aminoethyl))sulfonamide (dansyl ethylenediamine)
Molecular Probes™ BODIPY™ 530/550 NHS Ester (Succinimidyl Ester)
BODIPY™ 530/550 dye is bright, orange fluorescent dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.
Molecular Probes™ DNP-X, SE, 6-(2,4-Dinitrophenyl)aminohexanoic Acid, Succinimidyl Ester
DNP-X, SE, 6-(2,4-Dinitrophenyl)aminohexanoic Acid, Succinimidyl Ester
Molecular Probes™ BODIPY™ FL NHS Ester (Succinimidyl Ester)
BODIPY™ FL dye is bright, green fluorescent dye with similar excitation and emission to fluorescein (FITC) or Alexa Fluor™ 488 dye.
Molecular Probes™ BODIPY™ 576/589 NHS Ester (Succinimidyl Ester)
BODIPY™ 576/589 dye is bright, red fluorescent dye with similar excitation and emission to Rhodamine Red™ and Alexa Fluor™568.
Molecular Probes™ BODIPY™ 558/568 NHS Ester (Succinimidyl Ester)
BODIPY™ 558/568 dye is bright, red fluorescent dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.
Molecular Probes™ Dabcyl, SE, 4-((4-(dimethylamino)phenyl)azo)benzoic Acid, Succinimidyl Ester
4-((4-(dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester (dabcyl, SE)
Invitrogen™ CPM (7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin)
The thiol-reactive coumarin, CPM is very weakly fluorescent until reacted with thiols producing a conjugate with excitation/emission maxima of ∼384/470 nm.
Invitrogen™ Click-IT™ AHA (L-Azidohomoalanine)
Non-radioactive technique