Crosslinking Reagents
Thermo Scientificâ„¢ Pierceâ„¢ EDC, No-Weighâ„¢ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
MilliporeSigmaâ„¢ Calbiochemâ„¢ SATA
Reacts with primary amines to add protected sulfhydryl groups.
MilliporeSigmaâ„¢ Calbiochemâ„¢ THIOLYTEâ„¢ Monobromobimane Reagent, Fluorescent Labeling Compound
Used to label E.coli thioredoxin.
MilliporeSigmaâ„¢ Calbiochemâ„¢ SMCC, Water-Soluble
Used for coupling enzymes to antibodies.
Thermo Scientificâ„¢ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.
Thermo Scientificâ„¢ LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)
Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups at ends of 10-atom (15.7A) spacer arm.
Thermo Scientificâ„¢ SM(PEG)6 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ KMUH (N-κ-maleimidoundecanoic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (19.0A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ AMAS (N-α-maleimidoacet-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 4-atom (4.4A) spacer arm.
Thermo Scientific™ EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 8-atom (9.4A) spacer arm.
Thermo Scientificâ„¢ DTME (dithiobismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Thermo Scientificâ„¢ SIA (succinimidyl iodoacetate)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Thermo Scientificâ„¢ L-Photo-Methionine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
![Thermo Scientificâ„¢ SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)](https://assets.fishersci.com/TFS-Assets/LSG/product-images/23013-SPB.jpg-250.jpg)
Thermo Scientificâ„¢ SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)
Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.
Thermo Scientificâ„¢ DTBP (Wang and Richard's Reagent)
Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.