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Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

Supplier:  Thermo Scientific™ 21650

Catalog No. PI21650


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Description

Description

Thermo Scientific™ Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Highlights:

  • Forms cleavable disulfide bonds with sulfhydryls
  • Releases a detectable byproduct after reacting with free sulfhydryl groups; by measuring the release of pyridine-2-thione at 343nm, the reaction progression can be monitored
  • Reactive groups: sulfo-NHS ester and pyridyldisulfide
  • Reactive toward: amino and sulfhydryl groups
Specifications

Specifications

Amine-Sulfhydryl
Sulfo-LC-SPDP
No
Upon receipt store at -20°C protected from moisture.
Ambient
Chemical Labeling
Pyridyldisulfide, Sulfo-NHS Ester
Powder
Water
Yes
By Thiols
527.57
15.7 Å
No
Pierce
Heterobifunctional
Medium (10 to 30 Å)
50 mg
Standard
Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.