Oligonucleotides

MilliporeSigma™ Novagen™ Oligo(dT) primer

Used for first-strand synthesis of cDNAs, using poly-adenylated mRNA as template

Thermo Scientific™ Transcription Promoter Sequencing Primers

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Thermo Scientific™ Transcription Promoter Sequencing Primers

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Thermo Scientific™ Oligo(dT)18 Primers

Thermo Scientific™ Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

Thermo Scientific™ Transcription Promoter Sequencing Primers

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Thermo Scientific™ pJET1.2 Sequencing Primers

Primers for sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2 and for colony screening by PCR.

Thermo Scientific™ Transcription Promoter Sequencing Primers

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Thermo Scientific™ M13/pUC reverse sequencing primer (-46), 24-mer

Sequence DNA fragments inserted into the MCS of various pUC19 based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

Thermo Scientific™ Transcription Promoter Sequencing Primers

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Thermo Scientific™ M13/pUC sequencing primer (-46), 22-mer

Sequence DNA fragments inserted into the MCS of various pUC19 based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

Thermo Scientific™ Exo-Resistant Random Primer

Perform highly efficient random priming of DNA synthesis reactions with this mixture of single-stranded random oligonucleotides.

Thermo Scientific™ M13/pUC sequencing primer (-40), 17-mer

Sequence DNA fragments inserted into the MCS of various pUC19 based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

Thermo Scientific™ Primers for cDNA Synthesis

Optimze cDNA synthesis with these random hexamer primers, oligo(dT)18 primers and anchored oligo dT primers.

Thermo Scientific™ M13/pUC reverse sequencing primer (-26), 17-mer

Sequence DNA fragments inserted into the MCS of various pUC19-based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

Thermo Scientific™ M13/pUC sequencing primer (-20), 17-mer

Accurately sequence DNA with M13 pUC sequencing primers, single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends.

Promega Random and Oligo (dT) Primers DFS Item

Use for first-strand cDNA synthesis with a reverse transcriptase.

Promega™ Transcription Factor Consensus Oligonucleotides DFS Item

These oligonucleotides contain consensus DNA-binding sites for individual transcription factors and have 5′ OH blunt ends, allowing labeling to high specific activity with T4 PNK.

Invitrogen™ Random Primers DFS Item

Truly random primers suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates

Invitrogen™ Random Decamers (50μM) DFS Item

Provided at a stock concentration of 50μM, and functionally tested using the RETROscript™ Kit

Invitrogen™ Oligo (dT) Primer (50mM) DFS Item

Provided at a stock concentration of 50μM

Invitrogen™ M13 Forward (-20) DFS Item

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Invitrogen™ Bio-16-UTP (10mM) DFS Item

Ideal for use as substrates as part of in vitro transcription reactions

Invitrogen™ M13 Reverse DFS Item

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Invitrogen™ RNA Century™-Plus Marker Templates DFS Item

Contain mixtures of linearized plasmids ready for use as templates during in vitro transcription reactions for synthesis of labeled RNA molecular size standards

Invitrogen™ Anchored Oligo(dT) 20 Primer DFS Item

Primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC)

Invitrogen™ 5-(3-Aminoallyl)-dUTP (50mM) DFS Item

When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups

Invitrogen™ Lambda Hind III dsDNA Markers DFS Item

Ambion Lambda DNA is digested to completion with Hind III

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