Life Science Buffers

Alfa Aesar™ TRIS, 0.5M buffer soln., pH 8.4

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Alfa Aesar™ Glycine, 0.2M buffer soln., pH 2.5

CAS: 56-40-6 Molecular Formula: C2H5NO2 Molecular Weight (g/mol): 75.067 MDL Number: MFCD00008131 InChI Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N PubChem CID: 750 ChEBI: CHEBI:15428 IUPAC Name: 2-aminoacetic acid SMILES: C(C(=O)O)N

Alfa Aesar™ TRIS, 0.5M buffer soln., pH 8.6

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

MilliporeSigma™ BIS-Tris, ULTROL™ Grade, Calbiochem™,

CAS: 6976-37-0 Molecular Formula: C8H19NO5 Molecular Weight (g/mol): 209.242 InChI Key: OWMVSZAMULFTJU-UHFFFAOYSA-N Synonym: [bis(2-Hydroxyethyl)imino]-tris(hydroxymethyl)methane PubChem CID: 81462 ChEBI: CHEBI:41250 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CO)N(CCO)C(CO)(CO)CO

Alfa Aesar™ EPPS, 0.2M buffer soln., pH 7.0

CAS: 16052-06-5 Molecular Formula: C9H20N2O4S Molecular Weight (g/mol): 252.329 MDL Number: MFCD00006160 InChI Key: OWXMKDGYPWMGEB-UHFFFAOYSA-N Synonym: HEPPS PubChem CID: 85255 ChEBI: CHEBI:42298 IUPAC Name: 3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid SMILES: C1CN(CCN1CCCS(=O)(=O)O)CCO

Alfa Aesar™ Imidazole, 0.2M buffer soln., pH 6.0, low endotoxin

CAS: 288-32-4 Molecular Formula: C3H4N2 Molecular Weight (g/mol): 68.079 MDL Number: MFCD00005183 InChI Key: RAXXELZNTBOGNW-UHFFFAOYSA-N PubChem CID: 795 ChEBI: CHEBI:16069 IUPAC Name: 1H-imidazole SMILES: C1=CN=CN1

MilliporeSigma™ Tris, Hydrochloride, ULTROL™ Grade, Calbiochem™,

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: tris(Hydroxymethyl)aminomethane, HCl PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

TE buffer, (20X), pH 7.4, autoclaved, Alfa Aesar™

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 MDL Number: MFCD00236359 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

MilliporeSigma™ SSC Buffer, 20X Powder Pack, ULTROL™ Grade, Calbiochem™

Each pack provides an adequate amount of material to make 1L of 20X concentrate that can be diluted to a 1X solution containing 150mM NaCl, 15mM sodium citrate, pH 8.0

MilliporeSigma™ Bicine, ULTROL™ Grade, Calbiochem™,

CAS: 150-25-4 Molecular Formula: C6H13NO4 Molecular Weight (g/mol): 163.173 InChI Key: FSVCELGFZIQNCK-UHFFFAOYSA-N Synonym: N,N-bis-(2-Hydroxyethyl)glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: C(CO)N(CCO)CC(=O)O

Alfa Aesar™ Elution buffer, pH 8.0

For elution of His-tag proteins from nickel resins and columns

MilliporeSigma™ HEPES, Free Acid, Molecular Biology Grade, Calbiochem™,

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Alfa Aesar™ TRIS, 0.5M buffer soln., pH 8.5

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

MOPS, 99%, Alfa Aesar™

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: C1COCCN1CCCS(=O)(=O)O

Alfa Aesar™ BIS-TRIS propane, 0.2M buffer soln., pH 6.5

CAS: 64431-96-5 Molecular Formula: C11H26N2O6 Molecular Weight (g/mol): 282.337 MDL Number: MFCD00004689 InChI Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N PubChem CID: 125132 ChEBI: CHEBI:40947 IUPAC Name: 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO

MilliporeSigma™ Tris Buffer, 1.0 M, pH 8.0, Molecular Biology Grade, Calbiochem™,

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 1,3-propanediol, 2-amino-2-(hydroxymethyl)- PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Alfa Aesar™ Potassium phosphate, 0.2M buffer soln., pH 7.2

CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.084 MDL Number: MFCD00011401 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M PubChem CID: 516951 ChEBI: CHEBI:63036 IUPAC Name: potassium;dihydrogen phosphate SMILES: OP(=O)(O)[O-].[K+]

Alfa Aesar™ TRIS, 0.5M buffer soln., pH 7.5

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Alfa Aesar™ TRIS, 0.5M buffer soln., pH 8.0

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Alfa Aesar™ Sucrose, 80%, in MNE buffer, MES + NaCl + EDTA

CAS: 57-50-1 Molecular Formula: C12H22O11 Molecular Weight (g/mol): 342.297 MDL Number: MFCD00006626 InChI Key: CZMRCDWAGMRECN-UGDNZRGBSA-N PubChem CID: 5988 ChEBI: CHEBI:17992 IUPAC Name: (2R,3R,4S,5S,6R)-2-[(2S,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol SMILES: C(C1C(C(C(C(O1)OC2(C(C(C(O2)CO)O)O)CO)O)O)O)O

Alfa Aesar™ Imidazole, 0.5M buffer soln., pH 6.5

CAS: 288-32-4 Molecular Formula: C3H4N2 Molecular Weight (g/mol): 68.079 MDL Number: MFCD00005183 InChI Key: RAXXELZNTBOGNW-UHFFFAOYSA-N PubChem CID: 795 ChEBI: CHEBI:16069 IUPAC Name: 1H-imidazole SMILES: C1=CN=CN1

EPPS, 99%, for biochemistry, ACROS Organics™

CAS: 16052-06-5 Molecular Formula: C9H20N2O4S Molecular Weight (g/mol): 252.329 MDL Number: MFCD00006160 InChI Key: OWXMKDGYPWMGEB-UHFFFAOYSA-N Synonym: 3-[4-(2-Hydroxyethyl)-1-piperazine]propanesulfonic acid PubChem CID: 85255 ChEBI: CHEBI:42298 IUPAC Name: 3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid SMILES: C1CN(CCN1CCCS(=O)(=O)O)CCO

TAE (10X), TRIS + acetate + EDTA, Alfa Aesar™

Used for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis, used at various concentrations to study the mobility of DNA in solution.

MOPS sodium salt, 98%, ACROS Organics™

CAS: 71119-22-7 Molecular Formula: C7H14NNaO4S Molecular Weight (g/mol): 231.242 MDL Number: MFCD00064350 InChI Key: MWEMXEWFLIDTSJ-UHFFFAOYSA-M Synonym: 3-(4-Morpholino)propanesulfonic acid sodium salt PubChem CID: 3859613 IUPAC Name: sodium;3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CCCS(=O)(=O)[O-].[Na+]

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