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Standard PCR Products

An assortment of products including reagents, kits, and buffers, for standard polymerase chain reaction (pcr) protocols.
Standard PCR Reagents and Kits (112)
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Invitrogen™ Platinum™ SuperFi II DNA Polymerase  DFS Item

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.

Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
3

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl2  DFS Item

Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA) is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.

Shipping Condition Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Purity or Quality Grade LD (Low DNA)
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
4

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Reaction Speed Standard
Hot Start No
Overhang 3'-A
Concentration 5 U/μL
5

Invitrogen™ Platinum™ Taq DNA Polymerase  DFS Item

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Starting Material DNA
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
6

Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
GC-Rich PCR Performance Medium
Content And Storage • Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 μM each of dATP, dTTP, dCTP and dGTP

Master mix is supplied with nuclease-free water.
Store at -20°C.
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR
7

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type High-Fidelity DNA Polymerase
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Standard PCR,High-fidelity PCR
8

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Hot-start PCR,High-fidelity PCR
9

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Shipping Condition Dry Ice
Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or less
Reaction Format Ready to use master mix
Reaction Speed 15 to 30 sec./kb
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
10

Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
Polymerase Phusion High-Fidelity DNA Polymerase
GC-Rich PCR Performance High
Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion GC & HF Buffer
• dNTP mix (10 mM each)
• Control template (λ DNA) and DNA size standard
• 1.3 kb & 10 kb primers (4 μM each)
• 50 mM MgCl2 solution and DMSO

Store at –20°C.
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Product Type High-Fidelity PCR Kit
Reaction Speed Fast
Hot Start No
Overhang Blunt
For Use With (Application) Standard PCR,High-fidelity PCR
11

Thermo Scientific™ Phusion™ Plus DNA Polymerase

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.

Shipping Condition Room Temp or Wet Ice
Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or less
Reaction Format Separate components
Reaction Speed 15 to 30 sec./kb
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
12

Invitrogen™ AccuPrime™ Pfx SuperMix  DFS Item

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

13

Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity  DFS Item

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Shipping Condition Dry Ice
Polymerase AccuPrime Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 9X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
For Use With (Application) Hot-start PCR,High-fidelity PCR
Detection Method Primer-probe
14

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase  DFS Item

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
15

Invitrogen™ Platinum™ SuperFi II PCR Master Mixes  DFS Item

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR