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Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).
Amplify signal in Western blot, ELISA or other biotin-based detection methods with this exclusive, high signal-to-noise, peroxidase-conjugated, ready-to-use streptavidin.
Commonly used as a second step for detection of biotinylated antibodies by ELISA and western blot. Avidin binds to biotin with high affinity. [Note: Sodium azide is an inhibitor of horseradish peroxidase enzymatic activity and should not be added to the AV-HRP diluent.]
Target Molecule | Biotin |
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Form | Liquid |
Label or Dye | HRP (Horseradish Peroxidase) |
Product Line | eBioscienceâ„¢ |
Horseradish peroxidase-conjugated His·Tag monoclonal antibody used as single detection reagent
Shrimp Alkaline Phosphatase (SAP) is a high specific activity, heat-labile alkaline phosphatase purified from a recombinant source and originally isolated from Pandalus borealis (arctic shrimp).
Enzyme | Alkaline Phosphatase |
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Concentration | 1 unit/μL |
For Use With (Application) | Dephosphorylation of DNA/RNA |
Avidin horseradish peroxidase is commonly used as a second step for the detection of biotinylated antibodies.
Content And Storage | Store at 2°C to 8°C |
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Target Molecule | Biotin |
Formulation | Aqueous buffer, contains carrier protein/stabilizer, no sodium azide |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Immunohistochemical Staining |
Product Line | eBioscienceâ„¢ |
Used in detection of proliferating cells within fixed tissue samples or cell populations
Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).
Specially prepared peroxidase-conjugated NeutrAvidin protein for maximum signal amplification with high signal-to-noise ratio in a ready-to-use solution.
Specificity | Biotin |
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Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2°C to 8°C. |
Target Molecule | Biotin |
Form | Liquid |
Enzyme | HRP (Horseradish Peroxidase) |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA |
Product Line | Pierceâ„¢ |
Detection Method | Colorimetric, Chemiluminescence, Chemifluorescence |
Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.
Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.
Content And Storage | Note: EZ-Link Plus Activated Peroxidase contains 1-3 moles of aldehyde per mole of peroxidase. Storage: Upon receipt store product protected from moisture at -20°C. Product is shipped with an ice pack. |
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Form | Solid |
Label or Dye | HRP (Horseradish Peroxidase) |
Solubility | Water |
Reactive Moiety | Aldehyde |
Label Type | Enzyme Labeled |
Chemical Reactivity | Amine |
Product Line | EZ-Linkâ„¢ |
Purified calf-intestinal alkaline phosphatase (AP, alk-phos) enzyme for use in activity assays and conjugation to antibodies for ELISA and IHC.
Content And Storage | Store at 2–8°C. |
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Label or Dye | AP (Alkaline Phosphatase) |
For Use With (Application) | ELISA, Immunohistochemistry |
Product Line | Pierceâ„¢ |
Amplify signal and improve detection of biotinylated antibodies for IHC and other methods using this streptavidin conjugated with HRP polymers.