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Invitrogen™ Avidin Protein, HRP

Catalog No. PI29994
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2 mg
5 mg
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Catalog No. Quantity
PI29994 5 mg
PI21123 2 mg
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Catalog No. PI29994 Supplier Invitrogen™ Supplier No. 29994
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Description

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Highlights:

  • Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10

  • Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin

  • Affordable – significantly less expensive than recombinant streptavidin

  • ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)

  • Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays

Specifications

Quantity 5 mg
Target Molecule Biotin
Content And Storage Store at -20°C. Shipped at ambient temperature.
Specificity Biotin
Enzyme HRP (Horseradish Peroxidase)
Form Solid
For Use With (Application) Western Blotting, ELISA, Immunohistochemistry
Product Line Pierce
Label or Dye HRP (Horseradish Peroxidase)

For Research Use Only. Not for use in diagnostic procedures.