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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur
2

Thermo Scientific™ Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin

Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.

Binding Property Chelator Stable
3

Thermo Scientific™ HisPur™ Cobalt Spin Columns, 3 mL

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

4

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

5

Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Shipping Condition Wet Ice
Matrix Affinity Resin
Format Lyophilized Powder
Form Lyophilized Powder
Protein Form Recombinant
Product Type Peptides
For Use With (Application) Protein Purification and Isolation
Product Line Pierce
6

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.

Shipping Condition Wet Ice
Stationary Phase Anti-DYKDDDDK monoclonal antibodies
Product Type Affinity Resin
7

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

8

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Multi-species) Conjugate
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate consists of a 13 kDa recombinant single domain antibody fragment that specifically binds to the Fc part of IgG from multiple species.

Ligand Type Antibody
Type Conjugate
Target IgG (Fc region)
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
9

MilliporeSigma™ Novagen™ Ekapture™ Agarose

Intended for rapid affinity-based capture.

Type Agarose
Content And Storage 2°C to 8°C
10

Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Designed for method optimization and parameter screening

Buffer 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <700 cm/hr.
Support Type Highly cross-linked Agarose with Dextran surface extender
Height (English) 3.93 in.
11

Thermo Scientific™ POROS™ 50 OH Hydroxyl Activated Resin
GREENER_CHOICE

Thermo Scientific™ POROS™ Immobilized Hydroxyl Affinity Bulk Media. POROS OH is based on an hydroxylated functional group designed for the immobilization of proteins through activation with tresyl-chloride, divinyl-sulphone or cyanogen bromide.

Shipping Condition Room Temperature
Pore Size 500 to 10,000 Å
Stationary Phase Hydroxyl
Type Bulk Resin
Content And Storage Store at room temperature.
Form Solid
Product Line POROS
12

Thermo Scientific™ Pierce™ Anti-c-Myc Agarose

Purify recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates with this immunoprecipitation resin.

Stationary Phase Antibody
Type Resin
Target c-Myc-Tagged Protein
Format Bottle
Column Type Affinity, Agarose Resin
Product Line Pierce
13

Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Easily capture and affinity-purify binding partners of any biotin-labeled protein or other ligand using these streptavidin agarose beads and complete reagent set.

14

Thermo Scientific™ CarboxyLink™ Coupling Resin

Covalently immobilize peptide antigens and other carboxylate ligands for affinity purification using DADPA-activated agarose beads and EDC crosslinker.

15

Thermo Scientific™ SDS-Out™ SDS Precipitation Kit

Quickly remove SDS from small-volume (0.1 to 10mL) protein samples with this SDS precipitation kit.