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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin
Description
Thermo Scientific Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport, which is a rigid, hydrophilic, highly crosslinked, copolymeric, and porous resin with high coupling capacity.
For protein purification, resin is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or C-terminus. Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target proteins with elution buffer.
Features of Pierce Anti-DYKDDDDK Affinity Resin
- Specific—unique base beads and highly specific antibody minimize off-target binding (low non-specific binding)
- High purity—optimized bind-wash-elute protocol enables high purity
- High yield—special antibody conjugation method enables high yield
- Rapid—entire purification protocol typically takes less than 40 mins
- Economical—purification protocol allows multiple reuses
- Flexible—available in multiple formats including bulk loose resin and spin columns
Characteristics of Pierce Anti-DYKDDDDK Affinity Resin
- Composition: anti-DYKDDDDK antibody covalently attached to UltraLink Biosupport, a highly crosslinked rigid support
- Particle size: 50–80 μm
- pH stability: 1–13
- Operating temperature: 2–30°C; do not freeze
- Particle concentration: 50% slurry in phosphate buffered saline, 0.02% sodium azide, pH 7.5
- Binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (∼32 kDa/mL settled resin)
Available formats
- Resin slurries—2 mL, 10 mL, and 100 mL of 50% slurry in PBS with 0.02% sodium azide, pH 7.5
- Spin columns—10 mL spin columns containing 3 mL of settled resin, supplied at 6 mL 50% slurry
Specifications
Specifications
| Product Line | Pierce |
| Product Type | Affinity Resin |
| Quantity | 100 mL |
| Formulation | 50% suspension in PBS containing 0.02% Sodium azide |
| Content And Storage | 50 mL settled resin. 100 mL supplied as a 50% slurry in PBS containing 0.02% sodium azide. Store at 2–8°C. |
| Format | Liquid |
| Stationary Phase | Anti-DYKDDDDK monoclonal antibodies |
| Shipping Condition | Wet Ice |
| Form | Liquid |
| Matrix | Affinity Resin |
Frequently Asked Questions (FAQs)
Anti-DYKDDDDK Magnetic Agarose (Cat. Nos. A36797, A36798, A36799B) is offered as a 25% suspension (1 mL of 25% suspension = 0.25 mL of settled beads). UltraLink-based Anti-DYKDDDDK Affinity Resin (Cat. Nos, A36801, A36803, A36804) is offered as a 50% slurry (1 mL of 50% slurry = 0.5 mL of settled resin).
Here is the binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (˜32 kDa)/mL settled resin.
Yes. The UltraLink-based Anti-DYKDDDDH (“FLAG”) Affinity Resin (Cat. Nos. A36801, A36803, A36804) can be used for FPLC purification.
An enterokinase cleavage site behind the DYKDDDDK (“FLAG”) tag can allow complete removal of the DYKDDDDK (“FLAG”) tag leaving no additional amino acids. We offer EKMax Enterokinase (Cat. Nos. E18001 and E18002) that can be used for this purpose. Subsequently, the EKMax Enterokinase can be removed using EK-Away Resin (Cat. No. R18001), a resin conjugated with soybean trypsin inhibitor, which has high affinity for enterokinase.
For Research Use Only. Not for use in diagnostic procedures.