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  6. Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Catalog No. PIA36806
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5 x 5 mg
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PIA36805 5 mg
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Catalog No. PIA36806 Supplier Thermo Scientific™ Supplier No. A36806
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Description

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Pierce 3X DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.9 g/mol. For use, reconstitute to 1.5 mg/mL in PBS.


This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. At a 1.5 mg/mL concentration, complete release of tagged protein can be achieved in 5–10 minutes at ambient temperature.


Note: Pierce 3X DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat. No. 89891).

Specifications

Product Line Pierce
Product Type Peptides
Protein Form Recombinant
Quantity 5 x 5 mg
Content And Storage 5 x 5 mg lyophilized peptide. Store at -5 to -30°C.
Format Lyophilized Powder
Shipping Condition Wet Ice
For Use With (Application) Protein Purification and Isolation
Form Lyophilized Powder
Matrix Affinity Resin

For Research Use Only. Not for use in diagnostic procedures.