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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (711)
Protease Inhibitors and Phosphatase Inhibitors (282)
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Miscellaneous Immobilized Affinity Ligands (45)
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1 – 15 of 958 results
1

MilliporeSigma™ Calbiochem™ RIPK3 Inhibitor, GSK'872

A cell-permeable, selective inhibitor of RIP3-selective kinase with >1,000-fold selectivity over a vast majority of more than 300 other kinases.

2

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

3

Invitrogen™ ProBond™ Nickel-Chelating Resin  DFS Item

A nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.

Type Resin
Stationary Phase Nickel-Chelating
Content And Storage The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Product Line ProBond
4

MilliporeSigma™ Calbiochem™ Protease Inhibitor Cocktail Set V, EDTA-Free

A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases.

Content And Storage Hygroscopic
Form Lyophilized
Target Proteins Serine and cysteine proteases, but not metalloproteases
Solubility H2O
5

Thermo Scientific™ pT7CFE1-CHis Vector for Mammalian Cell-Free Protein Expression

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

6

MilliporeSigma™ Novagen™ PopCulture™ Reagent

Formulated to extract proteins from E. coli cells directly in culture medium.

Content And Storage 15°C to 30°C
7

MilliporeSigma™ Calbiochem™ Adenosine 3',5'-cyclic Monophosphate, N6,O2'-Dibutyryl-, Sodium Salt

This Dibutyryl-cAMP, CAS 16980-89-5, is a cell-permeable cAMP analog that preferentially activates PKA.

Content And Storage Hygroscopic
CAS 16980-89-5
Form Lyophilized
Target Proteins PKA
Solubility H2O
Color White
Purity 95% by HPLC
Molecular Formula C18H23N5O8P·Na
Formula Weight 491.4g/mol
8

Thermo Scientific™ MCF7 (+hIGF-1) Control Lysate (1 mg/mL)

Thermo Scientific™ MCF7 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway.

9

Thermo Scientific™ A549 (+hIGF-1) Control Lysate (1 mg/mL)

Thermo Scientific™ A549 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway.

10

Thermo Scientific™ Sodium Deoxycholate Detergent

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Content And Storage Store at -20°C
Form Powder
Product Type Detergent
Reagent Type Detergent (Pure)
11

Thermo Scientific™ 1-Step Human High-Yield IVT Kit

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

Expressed Protein Type Insoluble Protein
Expression System Cell-Free
Product Line 1-Step
12

Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.

14

MilliporeSigma™ Calbiochem™ Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free

Aprotinin, Bovine, Recombinant, Nicotiana sp, Animal-Free - CAS 9087-70-1, is a heat and acid-stable competitive, reversible inhibitor of serine proteases.

CAS 9087-70-1
Form Liquid
Target Proteins chymotrypsin
Formulation In 0.9% NaCl. Contains no animal-derived components.
Color Clear Colorless to Amber
Purity 95% by SDS-PAGE
Molecular Formula C284H432N84O79S7
Formula Weight 6512g/mol
15

MilliporeSigma™ Calbiochem™ Dicoumarol

A cell-permeable quinone reductase inhibitor with anticoagulant properties.