Modifying Enzymes
Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)
Invitrogen™ Anza™ Alkaline Phosphatase
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Applied Biosystems™ Exonuclease I, standard concentration, (10 units/μL)
Exonuclease I is particularly useful in preparing the products of PCR for applications involving sequencing or labeling methods.
Thermo Scientific Chemicals Pectinase, Aspergillus niger
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Invitrogen™ Bovine Serum Albumin
Suitable for use as enzyme stabilizer
MilliporeSigma™ RNase A
As supplied with Montage™ Plasmid MiniprepHTS 96 Kit
Invitrogen™ LR Clonase II for BaculoDirect™ Kits
Eukaryotic system for high-level protein expression in insect cells
Invitrogen™ Anza™ DNA Blunt End Kit
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ Micrococcal Nuclease (300 U/μL)
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.
MilliporeSigma™ Calbiochem™ Y-27632
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile
Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone.
Invitrogen™ Topoisomerase I
Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds