Modifying Enzymes
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
MilliporeSigma™ Calbiochem™ RNase A, Bovine Pancreas
For selective degradation of RNA
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Invitrogen™ Proteinase K Powder
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Thermo Scientific™ RNase A/T1 Mix
Get the RNA degradation activity of both RNase A and RNase T1 with Thermo Scientific™ RNase A/T1 Mix.
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile
Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone.
Applied Biosystems™ Exonuclease I, standard concentration, (10 units/μL)
Exonuclease I is particularly useful in preparing the products of PCR for applications involving sequencing or labeling methods.