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DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.

2

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

3

Invitrogen™ TrueTag™ Donor DNA Kit, GFP stem  DFS Item

The Invitrogen TrueTag Donor DNA Kit, GFP Stem, provides a rapid solution to drive high-efficiency tagging (up to 100% edited cells) of silent or differentially expressed genes with transfection-ready donor DNA.

4

Invitrogen™ TrueTag™ Donor DNA Kit, RFP stem  DFS Item

The Invitrogen TrueTag Donor DNA Kit, RFP Stem, provides a rapid solution to drive high-efficiency tagging (up to 100% edited cells) of silent or differentially expressed genes with transfection-ready donor DNA.

5

Invitrogen™ TrueTag™ Donor DNA Kit, GFP  DFS Item

The Invitrogen™ TrueTag™ Donor DNA Kit, GFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

6

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

7

Invitrogen™ TrueTag™ Donor DNA Kit, YFP  DFS Item

The Invitrogen TrueTag Donor DNA Kit, YFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

8

Invitrogen™ TrueTag™ Donor DNA Kit, Myc  DFS Item

The Invitrogen TrueTag Donor DNA Kit, Myc, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

9

Invitrogen™ TrueTag™ Donor DNA Kit, DDK  DFS Item

The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

10

Invitrogen™ TrueTag™ Donor DNA Kit, BFP  DFS Item

The Invitrogen TrueTag Donor DNA Kit, BFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

11

Thermo Scientific™ pUC57 DNA

Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.

12

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix  DFS Item

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

Shipping Condition Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 15 fragments
Product Type Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART
13

Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Product Type Rapid DNA Ligation Kit
Enzyme DNA Ligase
14

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

15

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.