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An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 15 fragments |
Product Type | Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
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Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.