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Standard PCR Reagents and Kits

Products for use during standard PCR procedures. Includes master mixes, DNA polymerases, reverse transcription mixes, PCR beads, buffers, and kits. Ideal when specificity; precise quantification; and high fidelity, sensitivity, and yield are not requirements.
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1 – 15 of 51 results
1

Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with or without protocol  DFS Item

The GeneAmp Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti 96-Well Thermal Cycler.

GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq DNA Polymerase
No. of Reactions 250 Reactions
Format Tube
Volume 0.1 mL or 0.2 mL
Size (Final Product) 2 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
2

Applied Biosystems™ CLA IdentiFiler™ Plus PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

3

Applied Biosystems™ Fast MicroSEQ™ D2 Fungal rDNA PCR Kit
GREENER_CHOICE

This kit is the PCR component of the FAST MicroSEQ Fungal Identification System, with enough reagents for 50 PCR amplifications of fungal DNA unknowns.

4

Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

5

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase  DFS Item
GREENER_CHOICE

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Fidelity (vs. Taq) 1X
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Overhang 3'-A
Concentration 5 U/μL
Starting Material DNA
Exonuclease Activity 5' - 3'
6

Applied Biosystems™ AmpliTaq Gold™ 360 Buffer Kit  DFS Item
GREENER_CHOICE

The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient.

7

Invitrogen™ Taq DNA Polymerase, native  DFS Item

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Standard PCR
Exonuclease Activity 5' - 3'
Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start No
Starting Material DNA
8

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Polymerase AmpliTaq DNA Polymerase
Fidelity (vs. Taq) 1X
Reaction Format Kit
Product Type PCR Buffer
Concentration 10X
For Use With (Application) Standard PCR
9

Applied Biosystems™ Fast MicroSEQ™ 500 16S rDNA PCR Kit  DFS Item

The Fast MicroSEQ™ 500 16S rDNA PCR kit utilizes new Fast technology and provides bacterial identifications from colony to results in as little as 5 hours using the Veriti™ 96-well Thermal Cycler.

10

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Content And Storage Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
11
Promotions Available

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe
12
Promotions Available

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe
13

Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer  DFS Item
GREENER_CHOICE

The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.

14

Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.

15

Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Reaction Format Separate Components
Product Type Taq DNA Polymerase (Recombinant)
Reaction Speed Standard
Hot Start No
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Standard PCR