
Real Time PCR Master Mixes
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Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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InhibitorToleranceSimple | Medium |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Multiplex Capability | Single-plex |
Detection Method | SYBR |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | PowerUPâ„¢, SYBRâ„¢ |
Applied Biosystemsâ„¢ Power SYBRâ„¢ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
GC-Rich PCR Performance | High |
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Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBRâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal PCR Master Mix, no AmpEraseâ„¢ UNG
TaqManâ„¢ Universal PCR Master Mix, No AmpEraseâ„¢ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, GeneAmp 9600, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqPathâ„¢ 1-Step Multiplex Master Mix
TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
Shipping Condition | Dry Ice |
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InhibitorToleranceSimple | High |
Concentration | 4X |
For Use With (Application) | Gene Expression,Microbial Detection |
PCR Method | 1-step RT-qPCR |
Multiplex Capability | Up to 4-plex |
Detection Method | Primer-probe |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Polymerase | Fast DNA Polymerase |
Sensitivity | Down to 10 copies |
Format | Tube |
Product Type | qPCR Master Mix |
Passive Reference Dye | Mustang Purple |
For Use With (Equipment) | QuantStudioâ„¢ 6 Pro Real-Time PCR System or QuantStudioâ„¢ 7 Pro, QuantStudioâ„¢ 3 or 5, QuantStudioâ„¢ 6/QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 12K Flex, QuantStudioâ„¢ Dx, QuantStudioâ„¢ Absolute Q Digital PCR System, ViiAâ„¢ 7, 7500 , 7500 Fast, 7500 Fast Dx |
Sample Type | DNA, RNA |
Reaction Speed | Fast or Standard |
Target Specificity | RNA only, Both RNA and DNA |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ TaqPathâ„¢ ProAmpâ„¢ Multiplex Master Mix
TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.
Shipping Condition | Wet Ice |
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Content And Storage | Store at 2°C to 8°C in the dark. |
InhibitorToleranceSimple | Very High |
For Use With (Application) | Genotyping,Pharmacogenomics |
PCR Method | qPCR |
Multiplex Capability | Up to 4-plex |
Detection Method | Primer-probe |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Polymerase | Taq DNA Polymerase |
Product Type | qPCR Master Mix |
Passive Reference Dye | Mustang Purple |
For Use With (Equipment) | QuantStudioâ„¢ 3 or 5, QuantStudioâ„¢ 6/QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 12K Flex, QuantStudioâ„¢ Dx, QuantStudioâ„¢ Absolute Q Digital PCR System, ViiAâ„¢ 7 Real-Time PCR System ViiAâ„¢ 7 Dx, 7500 Fast, StepOneâ„¢ or StepOnePlusâ„¢ |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ Absolute Qâ„¢ MAP16 Plate Kit and Absolute Qâ„¢ Universal DNA dPCR Master Mix
Eliminate the need to design custom assays and choose from over 20 million predesigned assays. Includes the Absolute Q MAP16 plate kit plus the Absolute Q Universal DNA dPCR master mix which is compatible with a wide range of applications and difficult targets.
Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green & Enduraâ„¢ 384-well Plates Bundle
Bundle and save up to 50% off PowerUp SYBR Green Master Mix and Plates
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Advanced Master Mix for qPCR, no UNG
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
InhibitorToleranceSimple | High |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Presence/Absence |
PCR Method | qPCR |
Multiplex Capability | Duplex |
Detection Method | Primer-probe |
Includes | dUTP |
Thermostability | 72 hr., Assembled Reaction |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 6 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 7 Pro, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
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InhibitorToleranceSimple | Very High |
For Use With (Application) | Microbial Detection |
PCR Method | qPCR |
Multiplex Capability | Up to 3-plex |
Detection Method | Primer-probe |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Sensitivity | Down to 1 copy |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiAâ„¢ 7 System, ViiA 7 Dx, QuantStudioâ„¢ Dx, StepOnePlusâ„¢ System, 7500 Fast Dx System, StepOneâ„¢ System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ Absolute Qâ„¢ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Applied Biosystemsâ„¢ Absolute Qâ„¢ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
Applied Biosystemsâ„¢ TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix, no Rox
TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
InhibitorToleranceSimple | Very High |
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For Use With (Application) | Microbial Detection |
PCR Method | qPCR |
Multiplex Capability | Up to 4-plex |
Detection Method | Primer-probe |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Sensitivity | Down to 1 copy |
Passive Reference Dye | None |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudioâ„¢ 12k Flex Dx, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiAâ„¢ 7 System, ViiA 7 Dx, QuantStudioâ„¢ Dx, StepOnePlusâ„¢ System, 7500 Fast Dx System, StepOneâ„¢ System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ Absolute Qâ„¢ MAP16 Plate Kit and 1-Step RT-dPCR Master Mix
A key component of the QuantStudio Absolute Q Digital PCR System which is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated sample separation.
Applied Biosystemsâ„¢ TaqPathâ„¢ DuraPlexâ„¢ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | Medium |
Content And Storage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
Form | Liquid |
InhibitorToleranceSimple | High |
Concentration | 4X |
For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
PCR Method | 1-step RT-qPCR |
Multiplex Capability | Up to 5-plex |
Detection Method | Primer-probe |
Includes | dUTP |
Thermostability | 8 hr., Assembled Reaction |
Polymerase | Applied Biosystemsâ„¢ AmpliTaqâ„¢ DNA Polymerase with Hot Start |
Format | Single-Tube |
Passive Reference Dye | ROX (Pre-mixed) |
Reaction Speed | Fast or Standard |
Target Specificity | RNA only, Both RNA and DNA |
Hot Start | Antibody-based |
Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ VetMAXâ„¢ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Polymerase | AmpliTaqâ„¢ Fast DNA Polymerase |
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Activation Time | 5 min. |
Final Product Type | Mastermix |
Form | Liquid |
Reaction Speed | Fast |
Label or Dye | ROX |
Hot Start | Built-in Hot Start |
Concentration | 2X |
Starting Material | DNA/RNA |
Compatibility | TaqManâ„¢ |
For Use With (Application) | Animal Health |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |