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  7. Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Catalog No. 4486295
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Catalog No. 4486295 Supplier Applied Biosystems™ Supplier No. 4486295

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

  • Optimized for multiplexing - validated for quadplex gene expression and genotyping assays
  • Best-in-class performance - superior sensitivity, accuracy, dynamic range, and specificity
  • Reduced run times on fast and standard instrumentation - optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
  • Seamless integration into your experiments - validated with Applied Biosystems™ TaqMan™ assays and TaqMan™ QSY™ probes for gene expression and genotyping experiments
  • Carry-over contamination control - contains heat-labile UDG
  • Broad instrument compatibility

Specifications

Concentration 2X
Content And Storage Contains 1 bottle (50 mL) of 2X TaqMan™ Multiplex Master Mix sufficient for 5000 reactions.


TaqMan™ Multiplex Master Mix contains AmpliTaq™ Fast DNA Polymerase, uracil-N glycosylase (UNG), dNTPs (with dUTP), MUSTANG PURPLE™ dye (passive reference), and optimized buffer.

Store in refrigerator (2–8°C).
Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method Multiplex qPCR
Polymerase AmpliTaq Fast DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System
Product Line TaqMan
Product Type Multiplex Master Mix
Quantity 50 mL
For Use With (Application) Gene Expression, Virus Detection
Fidelity (vs. Taq) 2 X
No. of Reactions 5000 Reactions
Sample Type DNA
Volume 50 mL
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Frequently Asked Questions (FAQs)

I need to multiplex but cannot use ROX. What options do I have?

For multiplex assays that include reporter dyes overlapping the ROX channel (i.e. JUN), we recommend using one of our optimized multiplex master mixes that include the passive reference dye, MUSTANG PURPLE. MUSTANG PURPLE is compatible with QuantStudio 5, 6, 7, and 12K Flex, 7500, 7500 Fast, and ViiA 7 real-time PCR instruments.

While not ideal, we also offer master mixes that contain no passive reference dyes. For selection help for master mixes with no passive reference, please contact Technical Support at techsupport@thermofisher.com.
What master mix should I use for multiplexing in my qPCR experiments?

The choice of master mix depends upon the number of assays in your reaction, the application, and instrument compatibility. Please refer to our real-time PCR master mixes and instrument compatibility chart (https://assets.thermofisher.com/TFS-Assets/LSG/brochures/master-mix-instrument-compatibility.pdf) prior to selecting one of the master mixes below:

Gene Expression Assays:
- Single, duplex, and triplex assays: TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mix
- Multiplexing up to 4 assays: TaqMan Multiplex Master Mix or TaqPath 1-Step Multiplex Master Mix

SNP Genotyping Assays:
- Single SNP Genotyping assay: TaqPath ProAmp Master Mix
- Duplex SNP Genotyping assays: TaqPath ProAmp Multiplex Master Mix, TaqMan Multiplex Master Mix

For Research Use Only. Not for use in diagnostic procedures.