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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

2

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

3

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Form Liquid
Product Line Restore
4

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)  DFS Item

Optimized, easy-to-use blocker and primary antibody diluent system

5
Promotions Available

Invitrogen™ iBind™ Flex Western Starter Kit  DFS Item

Allows better antigen-antibody interaction and solutions flow between the iBind™ Card and membrane for maximum interaction between protein and antibody

6
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Invitrogen™ iBind™ Flex Solution Kit  DFS Item

Detection kit for use with iBind™ Flex Western System

No. of Reactions 10 midi blots or 20 mini blots
Product Type Flex Solution Kit
For Use With (Equipment) iBind Flex Western Device
Membrane Compatibility Nitrocellulose, PVDF
Product Line iBind
Detection Method Colorimetric, Chromogenic, Chemiluminescence
7

Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)  DFS Item

All buffers are made with high-purity reagents and are strictly quality controlled

8

Invitrogen™ Incubation Tray, 10 x 14 cm  DFS Item

Enables convenient and even staining of E-PAGE™ blots

9

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
10

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007  DFS Item

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

12

Invitrogen™ ZOOM™ CHAPS  DFS Item

For use with ZOOM IEF Fractionator

13

Invitrogen™ iBind Flex PLUS Solution Kit

The iBind Flex PLUS Solution Kit increases signal sensitivity, requires less antibody per run, and simplifies western blotting immunoprocessing when used with the iBind Flex Western System. The iBind Flex PLUS Solution Kit comes with two ready to use solutions, iBind Flex PLUS Solution and iBind Flex PLUS Antibody Diluent.

Product Type Flex Solution Kit
15

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric