General Western Blot Reagents
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Thermo Scientificâ„¢ Restoreâ„¢ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
| Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restoreâ„¢ |
Thermo Scientificâ„¢ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
| Form | Liquid |
|---|---|
| Product Type | Western Blot Stripping Buffer |
| Concentration | 10X |
Invitrogenâ„¢ Novexâ„¢ Tris-Glycine SDS Buffer Kit
Made with high-purity reagents and are strictly quality controlled
Invitrogenâ„¢ Novexâ„¢ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371
Optimized for using with Novex pH 3-7 IEF gels
Invitrogenâ„¢ XCell IIâ„¢ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
Invitrogenâ„¢ NuPAGEâ„¢ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer
Invitrogenâ„¢ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
MilliporeSigmaâ„¢ ReBlot Plus Kit and Reagents
Blots can be stripped of antibodies in approximately 15 minutes at room temperature
| For Use With (Application) | Western Blot |
|---|
MilliporeSigmaâ„¢ Calbiochemâ„¢ ImmunoPenâ„¢
Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.
Invitrogenâ„¢ No-Stainâ„¢ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
| Shipping Condition | Dry Ice |
|---|---|
| Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
| Product Type | Protein Labeling Reagent |
| For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Quantity | 1 kit |
| Green Features | Less hazardous |
| Shelf Life | At least 2 years from receipt |
| Run Time | 10 minutes |
| For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
| Gel Compatibility | Any Gel Type and Gel Chemistry |
| Product Line | Invitrogenâ„¢ |
MilliporeSigmaâ„¢ Calbiochemâ„¢ MOWIOLâ„¢ 4-88 Reagent
Used for mounting cells on slides for detection of cell-surface antigens.
Invitrogenâ„¢ Boltâ„¢ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis