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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,957)
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1 – 15 of 1,263 results
1

Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns

Use as a robust, general-purpose column for QC/QA applications. They are high-quality, base-deactivated, fully endcapped, have similar C18 selectivity but slightly less retentive.

Pore Size 130 Å
Carbon Load 7%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L7
2

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å Biphenyl HPLC Column, 5 μm Particle Size

Ascentis™ Express 90 Å Biphenyl columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Biphenyl (Dimethylbiphenyl) Bonding
Phase Reversed Phase
Matrix Biphenyl (Dimethylbiphenyl) Bonding Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Surface Area 90 m2/g
Product Line Ascentis
3

MilliporeSigma™ Supelco™ Carbosieve™ SIII Thermal Desorption Tubes

Suitable for use with Air Monitoring

Type Desorption Tube
Matrix Carbosieve™ S-III Carbon Molecular Sieve
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Packaging Quantity 10/Pk.
Material Stainless Steel
Overall Length (English) 3.5 in.
For Use With (Application) Air Monitoring
Methods EPA TO-2
Product Line Carbosieve
4

TSKgel™ Alpha-M HPLC Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel Alpha-M Columns are so-called mixed-bed columns with an extended linear calibration range. They are packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in different solvent systems.

5

MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide HPLC Column, 2.7 μm Particle Size

Ascentis™ Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis™ Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from 'phase collapse'.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
6

TSKgel™ G4000HHR HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel G4000HHR Columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 400,000 Da.

7

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns
GREENER_CHOICE

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pore Size 120 Å
Carbon Load 8%
Stationary Phase Proprietary
Temperature 70°C
Phase HILIC
For Use With LC/MS
Endcapped Yes
Column Type HILIC
pH 2 to 8
Surface Area 300 m²/g
Product Line Acclaim
9

Thermo Scientific™ Hypersil™ Phenyl-2 HPLC Columns

Use as a general-purpose column with aromatic selectivity with low silanol binding.

Pore Size 120 Å
Particle Size 5 μm
Carbon Load 5%
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L11
10

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

11

Thermo Scientific™ POROS™ CaptureSelect™ FSH Affinity HPLC Columns

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates.

Pore Size 2000 Å
Stationary Phase Follicle Stimulating Hormone affinity ligand, humanspecific
Column Type Affinity
Quantity 1 Column
Material PEEK
For Use With (Application) FPLC
High-throughput Compatibility Automated Protocols
Max. Pressure 180 bar
Particle Size 20 μm
Target Molecule Follicle Stimulating Hormone
Product Type Column
Binding Capacity 1.5 mg/mL resin
Sample Type Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
Purification Target Follicle Stimulating Hormone
Product Line CaptureSelect, POROS
12

Thermo Scientific™ Acclaim™ Mixed Mode WAX-1 HPLC Columns
GREENER_CHOICE

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns.

Pore Size 120 Å
Phase Reversed Phase, Ion Exchange, HILIC
Column Type Mixed Mode
pH 2.5 to 7.5
Surface Area 300 m²/g
Product Line Acclaim
USP Type L78
13

MilliporeSigma™ Supelco™ Carbotrap™ 217 Thermal Desorption Tubes

Suitable for use with Air Monitoring

Matrix Carbotrap™ 217, Carbotrap™ B Graphitized Carbon Black (GCB), Carboxen™ 1000 Carbon Molecular Sieve (CMS)
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Packaging Quantity 10/Pk.
Overall Length (English) 3.5 in.
For Use With (Application) Air Monitoring
Methods EPA TO-14, EPA TO-17
Product Line Carbotrap
14

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 10 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
15

MilliporeSigma™ Supelco™ SRT™-C SEC-300 Gel Filtration Column, 5 μm Particle Size, Stainless Steel

The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

Pore Size 300 Å
Particle Size 5 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line SRT