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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,956)
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1 – 15 of 1,269 results
1

TSKgel™ G2000SW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G2000SW SEC columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.

Pore Size 125 Å
Particle Size 10 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 1.2 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 7.5 mm
pH 2.5 - 7.5
Product Line TSKgel
USP Type L20, L59
2

Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC Columns, 1.5μm

Use the 1.5μm solid core particles of these columns with the Vanquish UHPLC system for unique high-efficiency, high-resolution analyses.

Pore Size 80 Å
Particle Size 1.5 μm
Carbon Load 6.5%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
Diameter (Metric) 2.1 mm
pH 2 to 9
Surface Area 110 m²/g
USP Type L1
3

MilliporeSigma™ Supelco™ Proteomix™ SAX-NP5 Strong Anion Exchange Column, 5 μm Particle Size, Stainless Steel

Used for the analyis or small scale purification of negatively charged biopolymers, such as glycoprotein isoforms, pegylated proteins and oligonucleotide.

Pore Size Nonporous
Particle Size 5 μm
Stationary Phase Ammonium Ion, Quaternary
Phase Anion Exchange
Matrix Spherical PS-DVB/Ammonium Ion, Quaternary Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Anion Exchange HPLC
Product Line Proteomix
4

Thermo Scientific™ Accucore™ Phenyl-Hexyl HPLC Columns

Use this solid core column for fast, high-resolution separations of aromatic and moderately polar compounds.

Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 5%
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
Packing Material Ultrapure Silica
pH 2 to 8
Surface Area 130 m²/g
Particle Shape Solid Core
Max. Pressure 14500 psi (1000 bar)
Product Line Accucore
USP Type L11
5

Thermo Scientific™ Syncronis™ aQ C18 Polar Endcapped HPLC Columns

Use for predictable HPLC separations of compounds in highly aqueous mobile phases.

Pore Size 100 Å
Carbon Load 19%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Polar
Column Type Reversed Phase
pH 2 to 8
Surface Area 320 m²/g
USP Type L1
6

TSKgel™ Q-STAT HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Q-STAT columns are packed with hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered anion exchange groups.

7

TSKgel™ SuperH5000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous polymer beads and used for the non-aqueous separation of polymers with molecular weights less than 4,000,000 Da.

8

MilliporeSigma™ Supelco™ LiChrospher™ NH2 HPLC Column, 5 μm Particle Size

LiChrospher™ is a reliable and versatile spherical silica sorbent, which is traditionally produced and available with different modifications.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase Amino (NH2)
Phase Normal Phase, HLIC, Anion Exchange
Matrix Amino (NH2) Phase
Endcapped No
Surface Area 350 m2/g
Product Line LiChrospher
9

Thermo Scientific™ Hypersil GOLD™ Silica HPLC Columns

Efficiently separate nonpolar and moderately polar organic compounds by normal phase chromatography using Thermo Scientific™ Hypersil GOLD™ Silica LC Columns.

Pore Size 175 Å
Stationary Phase Silica
Temperature 60°C
Phase Normal Phase and HILIC
pH 2 to 8
Surface Area 220 m²/g
USP Type L3
10

Thermo Scientific™ POROS™ CaptureSelect™ hCG Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ hCG Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands.

Shipping Condition Wet Ice
Pore Size 2000 Å
Content And Storage Column includes EZ grip fittings and column test certificate.

Store at 2°C to 8°C. DO NOT FREEZE.
Stationary Phase Chorionic Gonadotropin affinity ligand, human‐specific
Temperature 2-40°C
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 2400 psi (170 bar)
Column Format Analytical Column, Biocompatible PEEK
Particle Size 20 μm
Packing Material Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Binding Capacity 2.7 mg/ml
pH 1-10
Technique Bio-LC
Product Line POROS CaptureSelect
11

MilliporeSigma™ Supelco™ CHIRALPAK™ HSA HPLC Column, 5 μm Particle Size

CHIRALPAK™ HSA, which uses human serum albumin as the chiral selector, is highly selective for acidic racemates, preferably weak and strong acids, zwitterionic and non-protolytic (neutral) compounds.

Particle Size 5 μm
Stationary Phase Human Serum Albumin
Phase Chiral
Matrix Human Serum Albumin Phase
Technique Chiral HPLC
Product Line CHIRALPAK
12

TSKgel™ SuperAW5000 HPLC Column, 7 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in different solvent systems. Used for the separation of polymers that are soluble in polar organic solvents. Exclusion Limit (DMF): Polyethylene Oxide 4,000,000 Da.

13

Thermo Scientific™ Accucore™ C8 HPLC Columns

Use these columns, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 5%
Stationary Phase C8
Temperature 70°C
Phase Reversed Phase
pH 2 to 9
Surface Area 130 m²/g
Product Line Accucore
USP Type L7
14

MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano UHPLC Column, 2 μm Particle Size

The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express 2 μm ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Pore Size 90 Å
Particle Size 2 μm
Stationary Phase Diisopropyl-Cyanopropylsilane
Phase Reversed Phase
Matrix Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform
Endcapped Yes
pH 1 to 8
Technique Fused-Core UHPLC
Product Line Ascentis
15

MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide HPLC Column, 5 μm Particle Size

Ascentis™ Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis™ Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from 'phase collapse'.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core HPLC
Product Line Ascentis