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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,947)
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1 – 15 of 1,262 results
1

TSKgel™ GMPW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ GMPW is a mixed bed scouting column for water-soluble linear polymers. The pore volume can be accessed by polymers ranging in molecular weight from 500 to 8,000,000 Da.

Particle Size 17 μm
Solvents ≤20% organic solvent
Stationary Phase Hydroxylated Methacrylate
Temperature 80°C
Phase Size Exclusion
Matrix Polymer Particle Platform
Diameter (Metric) 7.5 mm
pH 2.0 - 12
Product Line TSKgel
USP Type L38, L39
2

Thermo Scientific™ Hypersil GOLD™ Silica HPLC Columns

Efficiently separate nonpolar and moderately polar organic compounds by normal phase chromatography using Thermo Scientific™ Hypersil GOLD™ Silica LC Columns.

Pore Size 175 Å
Stationary Phase Silica
Temperature 60°C
Phase Normal Phase and HILIC
pH 2 to 8
Surface Area 220 m²/g
USP Type L3
3

TSKgel™ Alpha-5000 HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous polymer beads which show minimal shrinkage or swelling in different solvent systems. Columns are best used for the separation of polymers that are soluble in polar organic solvents.

4

MilliporeSigma™ Supelco™ Proteomix™ SCX-NP10 Strong Cation Exchange Column, 10 μm Particle Size, PEEK

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Sulfonic Acid
Phase Cation Exchange
Matrix Spherical PS-DVB/Sulfonic Acid Phase
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Proteomix
5

MilliporeSigma™ Supelco™ CHIRALPAK™ CBH HPLC Column, 5 μm Particle Size

CHIRALPAK™ CBH phase has cellobiohydrolase as the chiral selector immobilized on spherical 5 μm silica particles.

Particle Size 5 μm
Stationary Phase Cellobiohydrolase
Phase Chiral
Matrix Cellobiohydrolase Phase
Technique Chiral HPLC
Product Line CHIRALPAK
6

MilliporeSigma™ Supelco™ Hamilton PRP-C18 HPLC Column, 5 μm Particle Size, Stainless Steel

The Hamilton PRP-C18 HPLC column is designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Material Stainless Steel
Product Line Hamilton
7

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pore Size 100 Å
Carbon Load 16%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 9
Surface Area 320 m²/g
USP Type L1
8

MilliporeSigma™ Supelco™ Luer Lock, Non-Jacketed Liquid Chromatography Column, 10 cm

Constructed of borosilicate glass and are rated at a maximum pressure of 15 psi (1 bar).

Length (Metric) 10 cm
Column Type Chromatography
Material Borosilicate Glass
9

MilliporeSigma™ Supelco™ Astec™ CYCLOBOND™ I 2000 AC Chiral HPLC Column, 5 μm Particle Size

CYCLOBOND™ I 2000 AC is used primarily for aromatic alcohols or amines that are chiral on the alpha or beta carbon.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase Cyclodextrin, Beta-
Phase Chiral
Matrix Cyclodextrin, Beta- Phase/Silica Particle Platform
Diameter (Metric) 4.6 mm
Technique Chiral HPLC
Product Line Astec CYCLOBOND
10

MilliporeSigma™ Supelco™ Kromasil C8 HPLC Column, 100 Å, 10 μm Particle SIze

For small molecules and peptides.

Pore Size 100 Å
Particle Size 10 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase
Product Line Kromasil
11

MilliporeSigma™ Supelco™ SUPELCOSIL™ Suplex™ pKb-100 HPLC Column, 5 μm Particle Size

Suplex™ pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL™ LC-ABZ columns.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Silica Gel/Spherical Particle Platform
Endcapped No
Surface Area 170 m2/g
Product Line Suplex
12

MilliporeSigma™ Supelco™ BIOshell™ Glycan HPLC Columns, 2.7 μm Particle Size

BIOshell™ Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Hydroxy, Penta-, Spherical Silica Particle Platform
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase, Spherical Silica Particle Platform
Endcapped No
pH 2 to 9
Material Stainless Steel
Technique Fused-Core HPLC
Surface Area 135 m2/g
Product Line BIOshell
13

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-CN HPLC Column, 5 μm Particle Size

The LC-CN phase is often used as a substitute for silica because it offers the advantages of a bonded phase (such as quick equilibration, and less sensitivity to small changes of the water content in the mobile phase).

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase Cyano
Phase Reversed Phase
Matrix Cyano Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Diameter (Metric) 4.6 mm
Surface Area 170 m2/g
Product Line SUPELCOSIL
14

Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns
GREENER_CHOICE

Resolve polar and nonpolar compounds in a single run. These columns have high pH stability (pH 1.5–10) in the separation of basic, neutral, and acidic species.

Pore Size 120 Å
Stationary Phase Polar Embedded
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
Surface Area 300 m²/g
USP Type L60
15

Thermo Scientific™ POROS™ CaptureSelect™ hGH Affinty HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ hGH HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

Pore Size 2000 Å
Content And Storage Column includes EZ grip fittings and column test certificate.
Store at 2°C to 8°C. DO NOT FREEZE.
Stationary Phase Growth Hormone affinity ligand, human-specific
Column Type Affinity
Elution Volume Max 29 column volumes
Quantity 1 Column
Material PEEK
For Use With (Application) FPLC
High-throughput Compatibility Automated Protocols
Max. Pressure 2600 psi, 180 bar
Particle Size 20 μm
Type Column
Matrix POROS
Target Molecule Protein
Binding Capacity 1.7 mg/mL
Purification Target Protein
Elution Volume Min 14 column volumes
Product Line CaptureSelect, POROS