HPLC Columns and Cartridges
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Thermo Scientific™ Hypersil™ ODS C18 Column, 10μm
Provides an excellent C18 phase for a broad range of applications and a global standard for many existing methods.
| Pore Size | 120 Å |
|---|---|
| Particle Size | 10 μm |
| Carbon Load | 10% |
| Stationary Phase | C18 |
| Phase | Reversed Phase |
| Packing Material | Silica |
| Column Type | Reversed Phase |
| Diameter (Metric) | 4.6 mm |
| pH | 2 to 8 |
| Surface Area | 170 m²/g |
| USP Type | L1 |
| Column Format | Analytical Column |
MilliporeSigma™ Supelco™ Ascentis™ Express Phenyl-Hexyl HPLC Column, 2.7 μm Particle Size
Ascentis™ Express Phenyl-Hexyl phase columns have unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Stationary Phase | Phenyl-Hexyl |
| Phase | Reversed Phase |
| Matrix | Phenyl-Hexyl Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Discovery™ HS F5 HPLC Column, 3 μm Particle Size
The Discovery™ HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
| Pore Size | 120 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | PFP (Pentafluorophenyl) |
| Phase | Reversed Phase |
| Matrix | PFP (Pentafluorophenyl) Phase/Silica Gel, High Purity, Spherical Particle Platform |
| Endcapped | Yes |
| pH | 2 to 8 |
| Surface Area | 300 m2/g |
| Product Line | Discovery |
MilliporeSigma™ Supelco™ Ascentis™ Express Biphenyl Column, 2.7 μm Particle Size
The Ascentis™ Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Stationary Phase | Biphenyl |
| Phase | Reversed Phase |
| Matrix | Biphenyl Phase/Particle Platform (Fused-Core) |
| Endcapped | Yes |
| Material | Stainless Steel Hardware |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
Thermo Scientific™ Accucore™ Phenyl-X HPLC Columns
Use this solid core column for fast, high-resolution separations of aromatic compounds.
| Pore Size | 80 Å |
|---|---|
| Particle Size | 2.6 μm |
| Carbon Load | 5% |
| Temperature | 70°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Packing Material | Ultrapure Silica |
| pH | 2 to 8 |
| Surface Area | 130 m²/g |
| Max. Pressure | 14500 psi (1000 bar) |
Thermo Scientific™ Accucore™ C30 HPLC Columns
Use this specially designed solid core column for fast, high-resolution separations of hydrophobic, long-chain compounds.
| Pore Size | 150 Å |
|---|---|
| Particle Size | 2.6 μm |
| Carbon Load | 5% |
| Stationary Phase | C30 |
| Temperature | 70°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Packing Material | Ultrapure Silica |
| pH | 2 to 8 |
| Surface Area | 80 m²/g |
| Max. Pressure | 14500 psi (1000 bar) |
| Product Line | Accucore |
| USP Type | L62 |
MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C18 Column, 3.4 μm Particle Size (Dia. 75-300 μm)
BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core™ particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins.
| Particle Size | 3.4 μm |
|---|---|
| Stationary Phase | C18 (octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (octadecyl) Phase/Spherical Silica Particle Platform |
| Endcapped | Yes |
| pH | 1 to 9 |
| Material | Stainless Steel |
| Technique | Fused-Core HPLC |
| Surface Area | ̃15 m2/g |
| Product Line | BIOshell |
MilliporeSigma™ Supelco™ Antibodix™ WCX-NP5 Weak Cation Exchange Column, Stainless Steel, 5 μm Particle Size
Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.
| Pore Size | Nonporous |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Spherical PS-DVB |
| Phase | Cation Exchange |
| Matrix | Spherical PS-DVB Phase |
| pH | 2.0 to 12.0 |
| Material | Stainless Steel |
| Technique | Cation Exchange |
| Product Line | Antibodix |
MilliporeSigma™ Supelco™ Antibodix™ WCX-NP5 Weak Cation Exchange Column, PEEK, 5 μm Particle Size
Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.
| Pore Size | Nonporous |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Spherical PS-DVB |
| Phase | Cation Exchange |
| Matrix | Spherical PS-DVB Phase |
| pH | 2.0 to 12.0 |
| Material | PEEK |
| Technique | Cation Exchange |
| Product Line | Antibodix |
MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide UHPLC Column, 2 μm Particle Size
The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis™ Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis™ Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2 μm |
| Stationary Phase | Amide, Alkyl |
| Phase | Reversed Phase |
| Matrix | Amide, Alkyl Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| Technique | Fused-Core UHPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Ascentis™ Express HILIC UHPLC Column, 2 μm Particle Size
Contains a bare silica stationary phase that can be used for traditional normal phase separations or aqueous normal phase chromatography.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2 μm |
| Stationary Phase | Silica |
| Phase | Reversed Phase |
| Matrix | Silica Phase/Fused-Core Particle Platform |
| Endcapped | No |
| Technique | Fused-Core UHPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Ascentis™ Express Phenyl-Hexyl UHPLC Column, 2 μm Particle Size
Ascentis™ Express 2 μm Phenyl-Hexyl can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2 μm |
| Stationary Phase | Phenyl-Hexyl |
| Phase | Reversed Phase |
| Matrix | Phenyl-Hexyl Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| Technique | Fused-Core UHPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Nucleosil™ C18 HPLC Column, 3 μm Particle Size
Nucleosil™ C18 HPLC Column features nonpolar, endcapped phases for reversed-phase chromatography.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | C8 (Octyl) |
| Phase | Reversed Phase |
| Matrix | C8 (Octyl) Phase |
| Endcapped | No |
| Surface Area | 350 m2/g |
| Product Line | Nucleosil |
Thermo Scientific™ HyperREZ™ XP Carbohydrate H+ HPLC Columns
Manufactured with a proprietary process and subjected to stringent quality control procedures, these LC Columns are used for the appropriate counter-ion to meet application requirements.
| Particle Size | 8 μm |
|---|---|
| Phase | Ligand Exchange |
| For Use With | LC/MS |
| Packing Material | Spherical, Microporous Polymer |
| pH | 1 to 11 |
| Max. Pressure | 725 psi (50 bar) |