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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,956)
HPLC Guard Columns and Cartridges (551)
HPLC Guard Column Accessories (33)

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1 – 15 of 1,268 results
1

Thermo Scientific™ POROS™ CaptureSelect™ HSA Affinty HPLC Columns

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

Shipping Condition Wet Ice
Pore Size 2000 Å
Content And Storage Column includes EZ grip fittings and column test certificate

Store at 4°C. DO NOT FREEZE.
Stationary Phase Human Serum Albumin affinity ligand, humanspecific
Column Type Affinity
Quantity 1 Column
Material PEEK
High-throughput Compatibility Not High-throughput Compatible (Manual)
Max. Pressure 180 bar
Particle Size 20 μm
Flow Rate Up to 5000 cm/hr.
Type Column
Matrix Polystyrenedivinylbenzene
Reactivity Human
Target Molecule HSA, HSA Fusion Proteins
Binding Capacity 2.6 mg/mL
Sample Type Cell Culture Supernatants
Purification Target HSA, HSA Fusion Proteins
Product Line CaptureSelect, POROS
2

Thermo Scientific™ Hypersil™ Silica Normal Phase HPLC Column, 3 μm, 4.6 mm x 250 mm

Column Format: Analytical Column; Carbon Load: 0%; Pore Size: 120 Å; Column Phase: Normal Phase

3

Thermo Scientific™ EASY-Spray™ HPLC Columns

Robust capillary flow HPLC columns for sensitive protein, peptide and mAbs separation using the stand alone or integrated easy to use design.

Phase Reversed Phase
5

TSKgel™ PWXL Type Guard Column, 12 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Guard Columns are stand-alone and do not require separate holders. For use with 7.8 mm ID G2500PWxl-GMPWxl Columns, 12 μm.

6

TSKgel™ Super-Octyl HPLC Column, 2.3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Super-Octyl Columns are packed with monodispersed, 2 μm (nominal, mean particle size is 2.3 μm) spherical silica particles covalently bonded with octyl groups.

Particle Size 2.3 μm
Solvents 0-100% Organic Solvent
Carbon Load 5%
Stationary Phase C8 (octyl)
Temperature 10°C to 50°C
Phase Reversed Phase
Matrix C8 (octyl) Phase/Silica Particle Platform
pH 2 to 7.5
Surface Area 100 m2/g
Particle Shape Sphere
Product Line TSKgel
7

TSKgel™ SuperMP(PW)-H Guard Column, 12 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ SuperMultiporePW columns and guard columns can be used for water-soluble polymer analysis.

8

TSKgel™ Amide-80 HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

For the analysis of saccharides, glycans, oligosaccarides, peptides, and polar compounds from natural products.

Shipping Condition Shipped in 85% Acetonitrile, 15% Water
Pore Size 100 Å
Particle Size 5 μm
Solvents 0-100% Organic Solvent
Stationary Phase Carbamoyl
Temperature 10°C to 80°C
Matrix Carbamoyl Phase/Silica Particle Platform
pH 2 to 7.5
Technique HILIC
Surface Area 450 m2/g
Product Line TSKgel
USP Type L68
9

TSKgel™ G3000PWXL HPLC Column, 7 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Use TSKgel™ G3000PWXL columns for the analysis of water-soluble linear polymers with molecular weights up to 50,000 Da.

10

TSKgel™ Low Dead Volume Precolumn Filter with 0.5 μm Stainless Steel Frit, Sold by MilliporeSigma™ Supelco™

Can be used to protect the TSKgel columns. When working with columns that contain smaller than 5 μm particles, Tosoh Bioscience suggests the installation of a pre-column filter in front of the (guard) column to avoid clogging the top frit.

12

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 3 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
13

MilliporeSigma™ Supelco™ Kromasil C18 HPLC Column, 100 Å, 5 μm Particle Size

Kromasil C18 HPLC Column, 100 Å, 5 μm Particle Size for small molecules and peptides.

Pore Size 100 Å
Particle Size 5 μm
Type HPLC Column
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Packing Material Silica-based Monomeric
Product Line Kromasil
14

MilliporeSigma™ Supelco™ Ascentis™ C18 HPLC Column, 5 μm Particle Size

Ascentis™ C18 is an extremely stable and reliable HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
15

TSKgel™ ODS-100Z HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ ODS-100Z column's bonded phase is prepared by reacting the silica surface with a difunctional octadecylsilane reagent, followed by repeated endcapping with a monofunctional trimethylsilane reagent.

Particle Size 5 μm
Carbon Load 20%
Stationary Phase C18 (octadecyl)
Temperature 10°C to 50°C
Material Stainless Steel
Product Line TSKgel
USP Type L1