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Molecular Probes™ Click-iT™ EdU Alexa Fluor™ 555 HCS Assay DFS Item

Superior alternative to traditional proliferation assays

$682.00 - $2,835.00

Specifications

Color Orange
Detection Method Fluorescent
Dye Type Alexa Fluor 555
Excitation Wavelength Range 555/565
Format 96-well plate,96-well plate
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: The kit contains sufficient material for two 96-well plates.


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
C10353 Life Technologies
LSC10353
10 x 96 well plates Each for $2,835.00
C10352 Life Technologies
LSC10352
2 x 96 well plates Each for $682.00
Specifications

Description

The Click-iT EdU Alexa Fluor 555 HCS Assay is a superior alternative to traditional proliferation assays that is optimized for high-content imaging applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor dye in a fast, highly specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue. BrdU-labeled DNA is quantitated using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules. This step is time consuming and difficult to perform consistently. The harsh treatment can also adversely affect sample integrity and quality, which makes co-staining with other antibodies challenging.

  • Simple: works the first time, every time, in less time
  • Efficient: no denaturation steps or harsh treatment required
  • Gentle on samples: better preservation of cell morphology, antigen structure, and DNA integrity
  • Consistent: not dependent on variable antibody lots for detection
  • Less hazardous

Superior Proliferation Methodology
The Click-iT EdU Alexa Fluor 555 HCS Assay provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT EdU HCS assay is not only easy to use, but more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

Cell Analysis, Cell Cycle, Cell Proliferation, Cell Proliferation with Click Chemistry, Cell Viability, Proliferation and Function, Cellular Imaging, Click Chemistries, DNA Replication and Repair, High-Content Screening (HCS), Labeling Chemistry

Order Info

Shipping Condition: Room temperature

Description & Specifications

Specifications

Orange
Alexa Fluor 555
96-well plate,96-well plate
Store at 2° to 6°C, dessicate and protect from light.
Fluorescent
555/565
High Content Instrument